One-Step Microfluidic Fabrication of Polyelectrolyte Microcapsules in Aqueous Conditions for Protein Release.

Autor:	Zhang L; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA., Cai LH; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA., Lienemann PS; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA., Rossow T; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA., Polenz I; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA., Vallmajo-Martin Q; Department of Obstetrics, University Hospital Zurich, University of Zurich, Schmelzbergstr, 12, 8091, Zurich, Switzerland., Ehrbar M; Department of Obstetrics, University Hospital Zurich, University of Zurich, Schmelzbergstr, 12, 8091, Zurich, Switzerland., Na H; Alan G MacDiarmid Institute, College of Chemistry, Jilin University, Changchun, 130012 (C, hina., Mooney DJ; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA., Weitz DA; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA. weitz@seas.harvard.edu.
Jazyk:	angličtina
Zdroj:	Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2016 Oct 17; Vol. 55 (43), pp. 13470-13474. Date of Electronic Publication: 2016 Sep 26.
DOI:	10.1002/anie.201606960
Abstrakt:	We report a microfluidic approach for one-step fabrication of polyelectrolyte microcapsules in aqueous conditions. Using two immiscible aqueous polymer solutions, we generate transient water-in-water-in-water double emulsion droplets and use them as templates to fabricate polyelectrolyte microcapsules. The capsule shell is formed by the complexation of oppositely charged polyelectrolytes at the immiscible interface. We find that attractive electrostatic interactions can significantly prolong the release of charged molecules. Moreover, we demonstrate the application of these microcapsules in encapsulation and release of proteins without impairing their biological activities. Our platform should benefit a wide range of applications that require encapsulation and sustained release of molecules in aqueous environments. (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze:	MEDLINE
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