Toxicomorphomics and toxicokinetics of quinalphos on embryonic development of zebrafish (Danio rerio) and its binding affinity towards hatching enzyme, ZHE1.

Autor: Yashwanth B; Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-Indian Institute of Chemical Technology, Hyderabad, India., Pamanji R; Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India., Rao JV; Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India. Electronic address: jviict@gmail.com.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2016 Nov; Vol. 180, pp. 155-163. Date of Electronic Publication: 2016 Oct 01.
DOI: 10.1016/j.aquatox.2016.09.018
Abstrakt: This study outlines the toxic effects of Quinalphos (QP), an organophosphrous insecticide on the development of zebrafish (Danio rerio) embryos, with special emphasis on toxicomorphomics and toxicokinetics of target enzyme, AChE. A range of concentrations was used to elucidate the median lethal concentration (LC 50 ) of Quinalphos. Furthermore, embryos were exposed to two sub-lethal concentrations LC 10 (0.66mg/L) and LC 20 (1.12mg/L) along with a median lethal concentration (3.0mg/L) for 96h. Several morphological aberrations like lordosis, kyphosis, scoliosis, heart edema, breaks in the neuronal tube and underdeveloped facial parts were noticed, which were of concentration and time dependent. The QP has adequately hindered hatching process during the course of exposure which was upheld by the in silico docking studies with hatching enzyme, ZHE1. The length of hatchlings at 96h in LC 50 concentration was significantly reduced to 47% compared to control. A significant pericardial effusion (5 to 16 fold) was observed in >90% of LC 50 treated groups. Morphological changes in heart lead to the bradycardia, which ultimately leading to heart failure in some cases. The swimming behavior was significantly diminished in relation to the inhibition of AChE levels. From the in vitro kinetic studies, the kinetic constants K m , V max and inhibitory concentration K i (4.45×10 -5 M) was determined which supported the competitive nature of QP.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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