Detection and comparison of neuraminidase activities in human and bovine group B streptococci.

Autor: Ekin IH; Department of Microbiology, Faculty of Veterinary Medicine, University of Yuzuncu Yil, Van, Turkey., Gurturk K; Department of Microbiology, Faculty of Veterinary Medicine, University of Yuzuncu Yil, Van, Turkey., Ilhan Z; Department of Microbiology, Faculty of Veterinary Medicine, University of Yuzuncu Yil, Van, Turkey., Ekin S; Department of Biochemistry, Faculty of Science, University of Yuzuncu Yil, Van, Turkey., Borum AE; Department of Microbiology, Faculty of Veterinary Medicine, University of Balikesir, Balikesir, Turkey., Arabaci C; Department of Microbiology, Okmeydani Education and Research Hospital, Istanbul, Turkey., Yesilova A; Department of Biometry and Genetics, Faculty of Agriculture, University of Yuzuncu Yil, Van, Turkey.
Jazyk: angličtina
Zdroj: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2016 Dec; Vol. 124 (12), pp. 1093-1098. Date of Electronic Publication: 2016 Oct 06.
DOI: 10.1111/apm.12607
Abstrakt: Human and bovine group B streptococcus (GBS) isolates were serotyped and amounts of released N-acetylneuraminic acid from N-acetylneuraminyl-lactose by extracellular neuraminidase were colorimetrically assessed. According to serotyping by co-agglutination method, 30 of bovine GBS and 43 of human GBS could be serotyped (ST) by monospecific antisera coated with protein A. The remaining GBS strains were designated as nontypeable (NT). The released N-acetylneuraminic acid was determined in 90.9% of bovine GBS and 47.1% of human GBS isolates. The differences between the total bovine and human GBS isolates were statistically significant (p < 0.001). In comparison with detected N-acetylneuraminic acid level in bovine and human groups, significant decrease was observed in the bovine NT group according to increased human NT (p < 0.01) and bovine ST groups (p < 0.01). However, N-acetylneuraminic acid level in bovine ST and bovine total groups significantly (p < 0.001) increased with respect to the human ST group and human total group. Neuraminidase activity was detected more frequently in bovine GBS isolates. Considerable differentiations were observed between typeable and nontypeable isolates.
(© 2016 APMIS. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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