| Autor: |
Nimmakayala P; Gus R. Douglass Institute and Department of Biology, West Virginia State University Institute, WV, USA., Tomason YR; Gus R. Douglass Institute and Department of Biology, West Virginia State UniversityInstitute, WV, USA; Department of Selection and Seed Production, Dnepropetrovsk State Agrarian and Economic UniversityDnepropetrovsk, Ukraine., Abburi VL; Gus R. Douglass Institute and Department of Biology, West Virginia State University Institute, WV, USA., Alvarado A; Gus R. Douglass Institute and Department of Biology, West Virginia State University Institute, WV, USA., Saminathan T; Gus R. Douglass Institute and Department of Biology, West Virginia State University Institute, WV, USA., Vajja VG; Gus R. Douglass Institute and Department of Biology, West Virginia State University Institute, WV, USA., Salazar G; Department of Agriculture, Alcorn State University Lorman, MS, USA., Panicker GK; Department of Agriculture, Alcorn State University Lorman, MS, USA., Levi A; U.S. Vegetable Laboratory, United States Department of Agriculture, Agricultural Research Service Charleston, SC, USA., Wechter WP; U.S. Vegetable Laboratory, United States Department of Agriculture, Agricultural Research Service Charleston, SC, USA., McCreight JD; U.S. Agricultural Research Station Salinas, CA, USA., Korol AB; Department of Evolutionary and Environmental Biology, Haifa University Haifa, Israel., Ronin Y; Department of Evolutionary and Environmental Biology, Haifa University Haifa, Israel., Garcia-Mas J; Centre for Research in Agricultural Genomics, Consejo Superior de Investigaciones Científicas-Institute for Food and Agricultural Research and Technology-Universitat Autònoma de Barcelona-Universitat de Barcelona Barcelona, Spain., Reddy UK; Gus R. Douglass Institute and Department of Biology, West Virginia State University Institute, WV, USA. |
| Abstrakt: |
Melon ( Cucumis melo L.) is a phenotypically diverse eudicot diploid (2 n = 2 x = 24) has climacteric and non-climacteric morphotypes and show wide variation for fruit firmness, an important trait for transportation and shelf life. We generated 13,789 SNP markers using genotyping-by-sequencing (GBS) and anchored them to chromosomes to understand genome-wide fixation indices ( Fst ) between various melon morphotypes and genomewide linkage disequilibrium (LD) decay. The F ST between accessions of cantalupensis and inodorus was 0.23. The F ST between cantalupensis and various agrestis accessions was in a range of 0.19-0.53 and between inodorus and agrestis accessions was in a range of 0.21-0.59 indicating sporadic to wide ranging introgression. The EM (Expectation Maximization) algorithm was used for estimation of 1436 haplotypes. Average genome-wide LD decay for the melon genome was noted to be 9.27 Kb. In the current research, we focused on the genome-wide divergence underlying diverse melon horticultural groups. A high-resolution genetic map with 7153 loci was constructed. Genome-wide segregation distortion and recombination rate across various chromosomes were characterized. Melon has climacteric and non-climacteric morphotypes and wide variation for fruit firmness, a very important trait for transportation and shelf life. Various levels of QTLs were identified with high to moderate stringency and linked to fruit firmness using both genome-wide association study (GWAS) and biparental mapping. Gene annotation revealed some of the SNPs are located in β-D-xylosidase, glyoxysomal malate synthase, chloroplastic anthranilate phosphoribosyltransferase, and histidine kinase, the genes that were previously characterized for fruit ripening and softening in other crops. |
