| Autor: |
Rane LB; Department of Chemistry, Savitribai Phule Pune University, Pune - 411007, India. aak@chem.unipune.ac.in., Kate AN, Ramteke SN, Shravage BV, Kulkarni PP, Kumbhar AA |
| Jazyk: |
angličtina |
| Zdroj: |
Dalton transactions (Cambridge, England : 2003) [Dalton Trans] 2016 Nov 14; Vol. 45 (42), pp. 16984-16996. Date of Electronic Publication: 2016 Oct 03. |
| DOI: |
10.1039/c6dt02871c |
| Abstrakt: |
Two new zinc(ii) complexes, [Zn(l-His)(NIP)] + (1) and [Zn(acac) 2 (NIP)](2) (where NIP is 2-(naphthalen-1-yl)-1H-imidazo[4,5-f][1,10]phenanthroline, acac = acetyl acetone), have been synthesized and characterized by elemental analysis, UV-vis, fluorescence, IR, 1 H NMR and electron spray ionization mass spectroscopies. Gel retardation assay, atomic force microscopy and dynamic light scattering studies show that 1 and 2 can induce the condensation of circular plasmid pBR322 DNA into nanometer size particles under ambient conditions. Treatment of 2 with 5 mM EDTA restored 30% of the supercoiled form of DNA, revealing partial reversibility of DNA condensation. The in vitro transfection experiment demonstrates that the complexes can be used to deliver pCMV-tdTomato-N1 plasmid which expresses red fluorescent protein. The confocal studies show that the fluorescent nature of complexes is advantageous for visualizing the intracellular delivery of metal complexes as well as transfection efficiency using two distinct emission windows. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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