Fluorescence Imaging of Posterior Spiracles from Second and Third Instars of Forensically Important Chrysomya rufifacies (Diptera: Calliphoridae) .

Autor: Flores D; Laboratory of Immunology and Signal Transduction, Division of Natural Sciences and Mathematics, Chaminade University, 3140 Waialae Avenue, Honolulu, HI, 96816.; Graduate Program in Molecular Biosciences and Bioengineering, University of Hawaii at Mānoa, 1650 East-West Road, Honolulu, HI, 96816., Miller AL; Graduate Program in Molecular Biosciences and Bioengineering, University of Hawaii at Mānoa, 1650 East-West Road, Honolulu, HI, 96816., Showman A; Laboratory of Immunology and Signal Transduction, Division of Natural Sciences and Mathematics, Chaminade University, 3140 Waialae Avenue, Honolulu, HI, 96816.; Laboratory of Forensic Taphonomy, Division of Natural Sciences and Mathematics, Chaminade University, 3140 Waialae Avenue, Honolulu, HI, 96816., Tobita C; Laboratory of Immunology and Signal Transduction, Division of Natural Sciences and Mathematics, Chaminade University, 3140 Waialae Avenue, Honolulu, HI, 96816., Shimoda LM; Laboratory of Immunology and Signal Transduction, Division of Natural Sciences and Mathematics, Chaminade University, 3140 Waialae Avenue, Honolulu, HI, 96816., Sung C; Laboratory of Immunology and Signal Transduction, Division of Natural Sciences and Mathematics, Chaminade University, 3140 Waialae Avenue, Honolulu, HI, 96816., Stokes AJ; Laboratory of Experimental Medicine, John A. Burns School of Medicine, University of Hawaìi, 651 Ilalo Street, Honolulu, HI, 96813., Tomberlin JK; Department of Entomology, Texas A&M University, College Station, TX, 77843., Carter DO; Graduate Program in Molecular Biosciences and Bioengineering, University of Hawaii at Mānoa, 1650 East-West Road, Honolulu, HI, 96816., Turner H; Laboratory of Immunology and Signal Transduction, Division of Natural Sciences and Mathematics, Chaminade University, 3140 Waialae Avenue, Honolulu, HI, 96816.
Jazyk: angličtina
Zdroj: Journal of forensic sciences [J Forensic Sci] 2016 Nov; Vol. 61 (6), pp. 1578-1587. Date of Electronic Publication: 2016 Oct 05.
DOI: 10.1111/1556-4029.13189
Abstrakt: Entomological protocols for aging blowfly (Diptera: Calliphoridae) larvae to estimate the time of colonization (TOC) are commonly used to assist in death investigations. While the methodologies for analyzing fly larvae differ, most rely on light microscopy, genetic analysis, or, more rarely, electron microscopy. This pilot study sought to improve resolution of larval stage in the forensically important blowfly Chrysomya rufifacies using high-content fluorescence microscopy and biochemical measures of developmental marker proteins. We established fixation and mounting protocols, defined a set of measurable morphometric criteria and captured developmental transitions of 2nd instar to 3rd instar using both fluorescence microscopy and anti-ecdysone receptor Western blot analysis. The data show that these instars can be distinguished on the basis of robust, nonbleaching, autofluorescence of larval posterior spiracles. High-content imaging techniques using confocal microscopy, combined with morphometric and biochemical techniques, may therefore aid forensic entomologists in estimating TOC.
(© 2016 American Academy of Forensic Sciences.)
Databáze: MEDLINE
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