A hydrophobic ammonia-oxidizing archaeon of the Nitrosocosmicus clade isolated from coal tar-contaminated sediment.

Autor: Jung MY; Department of Microbiology, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju, 28644, South Korea., Kim JG; Department of Microbiology, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju, 28644, South Korea., Sinninghe Damsté JS; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, Utrecht University, P.O. Box 59, AB Den Burg, 1790, The Netherlands.; Department of Earth Sciences, Faculty of Geosciences, Utrecht University, P.O. Box 80.021, Utrecht, 3508, TA, The Netherlands., Rijpstra WI; Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, Utrecht University, P.O. Box 59, AB Den Burg, 1790, The Netherlands., Madsen EL; Department of Microbiology, Cornell University, Ithaca, NY, 14853-8101, USA., Kim SJ; Department of Microbiology, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju, 28644, South Korea., Hong H; Department of Microbiology, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju, 28644, South Korea., Si OJ; Department of Microbiology, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju, 28644, South Korea., Kerou M; Department of Genetics in Ecology, University of Vienna, Vienna, A-1090, Austria., Schleper C; Department of Genetics in Ecology, University of Vienna, Vienna, A-1090, Austria., Rhee SK; Department of Microbiology, Chungbuk National University, 1 Chungdae-ro, Seowon-Gu, Cheongju, 28644, South Korea.
Jazyk: angličtina
Zdroj: Environmental microbiology reports [Environ Microbiol Rep] 2016 Dec; Vol. 8 (6), pp. 983-992. Date of Electronic Publication: 2016 Oct 24.
DOI: 10.1111/1758-2229.12477
Abstrakt: A wide diversity of ammonia-oxidizing archaea (AOA) within the phylum Thaumarchaeota exists and plays a key role in the N cycle in a variety of habitats. In this study, we isolated and characterized an ammonia-oxidizing archaeon, strain MY3, from a coal tar-contaminated sediment. Phylogenetically, strain MY3 falls in clade 'Nitrosocosmicus' of the thaumarchaeotal group I.1b. The cells of strain MY3 are large 'walnut-like' cocci, divide by binary fission along a central cingulum, and form aggregates. Strain MY3 is mesophilic and neutrophilic. An assay of 13 C-bicarbonate incorporation into archaeal membrane lipids indicated that strain MY3 is capable of autotrophy. In contrast to some other AOA, TCA cycle intermediates, i.e. pruvate, oxaloacetate and α-ketoglutarate, did not affect the growth rates and yields of strain MY3. The attachment of cells of strain MY3 to XAD-7 hydrophobic beads and to the adsorbent vermiculite demonstrated the potential of strain MY3 to form biofilms. The cell surface was confirmed to be hydrophobic by the extraction of strain MY3 from an aqueous medium with p-xylene. Our finding of a strong potential for surface attachment by strain MY3 may reflect an adaptation to the selective pressures in hydrophobic terrestrial environments.
(© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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