Using Pseudomonas aeruginosa PAO1 to evaluate hydrogen peroxide as a biofouling control agent in membrane treatment systems.

Autor: Yang Y; Department of Civil and Environmental Engineering, National University of Singapore, Singapore, Singapore., Kitajima M; Division of Environmental Engineering, Hokkaido University, Sapporo, Japan., Pham TP; Department of Civil and Environmental Engineering, National University of Singapore, Singapore, Singapore., Yu L; Department of Civil and Environmental Engineering, National University of Singapore, Singapore, Singapore., Ling R; Department of Civil and Environmental Engineering, National University of Singapore, Singapore, Singapore.; NUS Environmental Research Institute, National University of Singapore, Singapore, Singapore., Gin KY; Department of Civil and Environmental Engineering, National University of Singapore, Singapore, Singapore.; NUS Environmental Research Institute, National University of Singapore, Singapore, Singapore., Reinhard M; Department of Civil and Environmental Engineering, National University of Singapore, Singapore, Singapore.; Department of Civil and Environmental Engineering, Stanford University, Stanford, CA, USA.
Jazyk: angličtina
Zdroj: Letters in applied microbiology [Lett Appl Microbiol] 2016 Dec; Vol. 63 (6), pp. 488-494. Date of Electronic Publication: 2016 Nov 02.
DOI: 10.1111/lam.12674
Abstrakt: Hydrogen peroxide (H 2 O 2 ) is widely used in water treatment for biofouling control and, in conjunction with catalysts, as a powerful oxidant for contaminant destruction. H 2 O 2 could potentially serve as an antifouling agent in reverse osmosis systems in lieu of chlorine-based disinfectants. The dependence of the biocidal efficiency of H 2 O 2 on cell density, temperature and H 2 O 2 concentration by determining the growth, attachment and viability of the model bacterium Pseudomonas aeruginosa PAO1 was studied. For controlling growth of planktonic PAO1 cells, the minimally required H 2 O 2 concentration depends on the cell density and temperature. The effect of H 2 O 2 to remove the existing biofilm was found to be effective in the presence of a high concentration bicarbonate (8·4 g l -1 ), which forms peroxymonocarbonate, a strong oxidant and disinfectant. Treatment with H 2 O 2 -bicarbonate reduced the density of live PAO1 cells, removed extracellular polymeric substances and lowered the average biofilm thickness while maintaining the integrity of the membrane, suggesting that this type of treatment may be a suitable 'in-place-cleaning' procedure for biofouled membranes.
Significance and Impact of the Study: H 2 O 2 is evaluated as a potential replacement for chlorine to control biofouling in membrane-based water treatment systems. The biocidal efficacy of H 2 O 2 was evaluated as a function of H 2 O 2 concentration, cell density and temperature using the model organism Pseudomonas aeruginosa PAO1. Results demonstrated that at low temperatures and low cell densities, bacterial growth and membrane biofouling can be prevented by low H 2 O 2 concentrations, and existing biofilms could be removed by H 2 O 2 -bicarbonate mixtures. Findings suggested that H 2 O 2 could be used as a low cost agent for prevention and controlling biofouling in reverse osmosis applications.
(© 2016 The Society for Applied Microbiology.)
Databáze: MEDLINE
Externí odkaz: