| Autor: |
Olovnikov IA; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia. allakalm@img.ras.ru., Morgunova VV, Mironova AA, Kordyukova MY, Radion EI, Olenkina OM, Akulenko NV, Kalmykova AI |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2016 Sep; Vol. 81 (9), pp. 1023-30. |
| DOI: |
10.1134/S000629791609011X |
| Abstrakt: |
The telomere is a nucleoprotein complex at the ends of linear chromosomes that protects them from fusion and degradation. The telomere consists of telomeric DNA, a protective protein complex and telomeric RNA. Biogenesis of telomeric transcripts in development is still far from being understood. Drosophila telomeres are elongated by a transposition of specialized telomeric retrotransposons that encode proteins. Using transgenic constructs encoding tagged telomeric protein, we found that transcripts of Drosophila telomeric element HeT-A bind Gag-HeT-A protein encoded by these transcripts. Maternal HeT-A transcripts and Gag-HeT-A form ribonucleoprotein granules around centrosomes, centers of microtubule organization, during blastoderm formation, upon disruption of telomere silencing during oogenesis. The specific localization of HeT-A RNA is dependent on microtubules since disruption of microtubules caused delocalization of HeT-A transcripts. This transgenic system is a valuable model for the study of telomeric RNA biogenesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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