POTENTIAL CROSS-CONTAMINATION OF SIMILAR Giardia duodenalis ASSEMBLAGE IN CHILDREN AND PET DOGS IN SOUTHERN BRAZIL, AS DETERMINED BY PCR-RFLP.
| Autor: | Quadros RM; Universidade do Planalto Catarinense (UNIPLAC), Faculdade de Ciências Biológicas e Biomedicina. Lages, SC, Brazil., Weiss PH; Universidade do Estado de Santa Catarina (CAV/UDESC), Laboratório de Bioquímica de Hemoparasitas e Vetores do Centro de Ciências. Lages, SC, Brazil., Marques SM; Universidade Federal do Rio Grande do Sul (UFRGS), Departamento de Patologia Clínica Veterinária da Faculdade de Veterinária. Porto Alegre, RS, Brazil., Miletti LC; Universidade do Estado de Santa Catarina (CAV/UDESC), Laboratório de Bioquímica de Hemoparasitas e Vetores do Centro de Ciências. Lages, SC, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2016 Sep 22; Vol. 58, pp. 66. Date of Electronic Publication: 2016 Sep 22. |
| DOI: | 10.1590/S1678-9946201658066 |
| Abstrakt: | Giardia duodenalis is an enteric parasite that has distinct genetic groups. Human infections are mainly caused by assemblages A and B, although sporadic infections by assemblages C and D have also been reported. Animals can be infected by a wide range of assemblages (A to H). The aim of this study is to identify the assemblages and sub-assemblages of G. duodenalis with zoonotic features in fecal samples of school-aged children, and in dogs that coexist in the same households in Lages, Santa Catarina, Brazil. Fecal samples of 91 children and 108 dogs were obtained and G. duodenalis cysts were detected in samples from 11 (12.08%) children and 10 (9.25%) dogs. DNA extracted from the 21 positive samples was analyzed by PCR-RFLP, using the gdh gene. Results showed the presence of sub-assemblages AI (2/11), AII (4/11), BIII (2/11), and BIV(3/11) among children and AI (5/10) and BIV(3/10) in dogs, with zoonotic characteristics, and the carnivore specific assemblage C (2/10). G. duodenalis was found to infect both children and dogs living in the same household, with the same sub-assemblage (BIV) indicating that pet dogs are a potential risk of transmission of G. duodenalis to humans. Competing Interests: CONFLICTING INTERESTS There are no conflicts of interest. |
| Databáze: | MEDLINE |
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