Sensitive inexpensive HPLC determination of four antiepileptic drugs in human plasma: application to PK studies.
| Autor: | Ibrahim FA; Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt., El-Yazbi AF; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt., Barary MA; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt., Wagih MM; Forensic Medicine Authority, Egyptian Ministry of Justice, El Sayeda Zeinab, Zeinhom 11628, Cairo, Egypt. |
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| Jazyk: | angličtina |
| Zdroj: | Bioanalysis [Bioanalysis] 2016 Nov; Vol. 8 (21), pp. 2219-2234. Date of Electronic Publication: 2016 Sep 28. |
| DOI: | 10.4155/bio-2016-0211 |
| Abstrakt: | Aim: Recently, polytherapy regimen has been introduced for the treatment of epileptic patients for better seizure control with lesser side effects and better control of multiple seizure types. Methodology: A simple, sensitive and highly specific reversed-phase HPLC method was developed for simultaneous determination of four antiepileptic drugs (AEDs), levetiracetam, lamotrigine, oxcarbazepine and carbamazepine, in real human plasma without interference from endogenous components of plasma. Conclusion: The method was proved to be linear in the range of 0.5-50 µg/ml for all drugs. It was successfully applied for clinical PK study of the AEDs in healthy volunteers following single administration. Also, this method was applied for simultaneous determination of the studied drugs in volunteers' plasma receiving synergistic binary combinations from the four AEDs when used as add-on therapy. The good precision and selectivity of the developed method allow it to be used for routine therapeutic drug monitoring of such drugs as a useful tool in epilepsy management. |
| Databáze: | MEDLINE |
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