Virus-Induced Gene Silencing Identifies an Important Role of the TaRSR1 Transcription Factor in Starch Synthesis in Bread Wheat.

Autor: Liu G; The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. liuguoyu@henau.edu.cn., Wu Y; The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. yufangwu628@henau.edu.cn.; The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. yufangwu628@henau.edu.cn., Xu M; The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. mengjunxu920304@henau.edu.cn.; The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. mengjunxu920304@henau.edu.cn., Gao T; The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. gaotian1211@henau.edu.cn.; The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. gaotian1211@henau.edu.cn., Wang P; The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. wangpf@henau.edu.cn.; The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. wangpf@henau.edu.cn., Wang L; The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. hnndlina@henau.edu.cn.; The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. hnndlina@henau.edu.cn., Guo T; The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. gtcwheat@henau.edu.cn.; The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. gtcwheat@henau.edu.cn., Kang G; The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. guozhangkang@henau.edu.cn.; The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. guozhangkang@henau.edu.cn.; The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. guozhangkang@henau.edu.cn.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2016 Sep 23; Vol. 17 (10). Date of Electronic Publication: 2016 Sep 23.
DOI: 10.3390/ijms17101557
Abstrakt: The function of a wheat starch regulator 1 (TaRSR1) in regulating the synthesis of grain storage starch was determined using the barley stripe mosaic virus-virus induced gene-silencing (BSMV-VIGS) method in field experiments. Chlorotic stripes appeared on the wheat spikes infected with barley stripe mosaic virus-virus induced gene-silencing- wheat starch regulator 1 (BSMV-VIGS-TaRSR1) at 15 days after anthesis, at which time the transcription levels of the TaRSR1 gene significantly decreased. Quantitative real-time PCR was also used to measure the transcription levels of 26 starch synthesis-related enzyme genes in the grains of BSMV-VIGS-TaRSR1-silenced wheat plants at 20, 27, and 31 days after anthesis. The results showed that the transcription levels of some starch synthesis-related enzyme genes were markedly induced at different sampling time points: TaSSI, TaSSIV, TaBEIII, TaISA1, TaISA3, TaPHOL, and TaDPE1 genes were induced at each of the three sampling time points and TaAGPS1-b, TaAGPL1, TaAGPL2, TaSSIIb, TaSSIIc, TaSSIIIb, TaBEI, TaBEIIa, TaBEIIb, TaISA2, TaPHOH, and TaDPE2 genes were induced at one sampling time point. Moreover, both the grain starch contents, one thousand kernel weights, grain length and width of BSMV-VIGS-TaRSR1-infected wheat plants significantly increased. These results suggest that TaRSR1 acts as a negative regulator and plays an important role in starch synthesis in wheat grains by temporally regulating the expression of specific starch synthesis-related enzyme genes.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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