| Autor: |
Portela LV; Departamento de Bioquímica, ICBS, UFRGS. Programa de Pós Graduação em Ciências Biológicas- Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, RS, CEP 90035-003, Brazil., Brochier AW; Departamento de Bioquímica, ICBS, UFRGS. Programa de Pós Graduação em Ciências Biológicas- Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, RS, CEP 90035-003, Brazil., Haas CB; Departamento de Bioquímica, ICBS, UFRGS. Programa de Pós Graduação em Ciências Biológicas- Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, RS, CEP 90035-003, Brazil., de Carvalho AK; Departamento de Bioquímica, ICBS, UFRGS. Programa de Pós Graduação em Ciências Biológicas- Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, RS, CEP 90035-003, Brazil., Gnoato JA; Departamento de Bioquímica, ICBS, UFRGS. Programa de Pós Graduação em Ciências Biológicas- Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, RS, CEP 90035-003, Brazil., Zimmer ER; Departamento de Bioquímica, ICBS, UFRGS. Programa de Pós Graduação em Ciências Biológicas- Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, RS, CEP 90035-003, Brazil.; Instituto do Cérebro (InsCer) do Rio Grande do Sul, Pontifícia Universidade Católica do Rio grande do Sul, Av. Ipiranga, 6690 - Bairro Jardim Botânico, Porto Alegre, RS, CEP: 90610.000, Brazil., Kalinine E; Departamento de Bioquímica, ICBS, UFRGS. Programa de Pós Graduação em Ciências Biológicas- Bioquímica, Rua Ramiro Barcelos, 2600 anexo, Porto Alegre, RS, CEP 90035-003, Brazil., Pellerin L; Département de Physiologie, Université de Lausanne, Rue du Bugnon 7, 1005, Lausanne, Switzerland., Muller AP; Unidade de Ciências da Saúde, Laboratório de Bioquímica e Fisiologia do Exercício Universidade do Extremo Sul Catarinense-UNESC, Av. Universitária, 1105 - Bairro Universitário, Criciúma, Santa Catarina, CEP: 88806-000, Brazil. alexandrep.muller@gmail.com. |
| Abstrakt: |
Hyperpalatable diets (HP) impair brain metabolism, and regular physical exercise has an apparent opposite effect. Here, we combined a prior long-term exposure to HP diet followed by physical exercise and evaluated the impact on some neuroenergetic components and on cognitive performance. We assessed the extracellular lactate concentration, expression of monocarboxylate transporters (MCTs), pyruvate dehydrogenase (PDH), and mitochondrial function in the hippocampus. Male C57BL/6J mice were fed 4 months with HP or a control diet. Subsequently, they were divided in the following groups: control diet sedentary (CDS), control diet exercise (CDE), HP diet sedentary (HPS), and HP diet exercise (HPE) (n = 15 per group) and were engaged for an additional 30-day period of voluntary exercise and HP diet. Relative to the control situation, exercise increased MCT1, MCT4, and PDH protein levels, while the HP diet increased MCT1 and MCT4 protein levels. The production of hydrogen peroxide (H 2 O 2 ) and the mitochondrial membrane potential (∆Ѱ m ) stimulated by succinate in hippocampal homogenates were not significantly different between groups. ADP phosphorylation and the maximal respiratory rate induced by FCCP showed similar responses between groups, implying a normal mitochondrial function. Also, extracellular brain lactate levels were increased in the HPE group compared to other groups soon after performing the Y-maze task. However, such enhanced lactate levels were not associated with improved memory performance. In summary, hippocampal protein expression levels of MCT1 and 4 were increased by physical exercise and HP diet, whereas PDH was only increased by exercise. These observations indicate that a hippocampal metabolic reprogramming takes place in response to these environmental factors. |
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