| Autor: |
Higashi T; Faculty of Pharmaceutical Sciences, Tokyo University of Science., Yokota M; Faculty of Pharmaceutical Sciences, Tokyo University of Science., Goto A; Faculty of Pharmaceutical Sciences, Tokyo University of Science., Komatsu K; Shizuoka Saiseikai General Hospital., Sugiura T; Shizuoka Saiseikai General Hospital., Ogawa S; Faculty of Pharmaceutical Sciences, Tokyo University of Science., Satoh M; Divisions of Clinical Mass Spectrometry, Chiba University Hospital., Nomura F; Divisions of Clinical Mass Spectrometry, Chiba University Hospital. |
| Jazyk: |
angličtina |
| Zdroj: |
Mass spectrometry (Tokyo, Japan) [Mass Spectrom (Tokyo)] 2016; Vol. 5 (Spec Iss), pp. S0051. Date of Electronic Publication: 2016 Aug 31. |
| DOI: |
10.5702/massspectrometry.S0051 |
| Abstrakt: |
A method for the simultaneous determination of 25-hydroxyvitamin D3 [25(OH)D3] and its 3-sulfate [25(OH)D3S] in newborn plasma, which is expected to be helpful in the assessment of the vitamin D status, using stable isotope-dilution liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) has been developed and validated. The plasma was pretreated based on the deproteinization and solid-phase extraction, then subjected to derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD). The derivatization enabled the accurate quantification of 25(OH)D3 without interference from 3-epi-25(OH)D3 and also facilitated the simultaneous determination of the two metabolites by LC/positive ESI-MS/MS. Quantification was based on the selected reaction monitoring with the characteristic fragmentation of the DAPTAD-derivatives during MS/MS. This method was reproducible (intra- and inter-assay relative standard deviations of 7.8% or lower for both metabolites) and accurate (analytical recovery, 95.4-105.6%). The limits of quantification were 1.0 ng/mL and 2.5 ng/mL for 25(OH)D3 and 25(OH)D3S, respectively, when using a 20-μL sample. The developed method was applied to the simultaneous determination of plasma 25(OH)D3 and 25(OH)D3S in newborns; it was recognized that the plasma concentration of 25(OH)D3S is significantly higher than that of 25(OH)D3, and preterm newborns have lower plasma 25(OH)D3S concentrations than full-term newborns. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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