Development and evaluation of a multiplex real-time PCR for the detection of IMP, VIM, and OXA-23 carbapenemase gene families on the BD MAX open system.

Autor: Teo JW; National University Hospital, Department of Laboratory Medicine, Division of Microbiology, Singapore, 119074, Republic of Singapore. Electronic address: Jeanette_Teo@nuhs.edu.sg., La MV; National Public Health Laboratory, Ministry of Health, 3 Biopolis Drive, Synapse #05-14/16, Singapore, 138623, Republic of Singapore., Lin RT; National University Hospital, Department of Laboratory Medicine, Division of Microbiology, Singapore, 119074, Republic of Singapore; National Public Health Laboratory, Ministry of Health, 3 Biopolis Drive, Synapse #05-14/16, Singapore, 138623, Republic of Singapore.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2016 Dec; Vol. 86 (4), pp. 358-361. Date of Electronic Publication: 2016 Aug 26.
DOI: 10.1016/j.diagmicrobio.2016.08.019
Abstrakt: A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all PCR processes including sample extraction to PCR resulting. A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates. This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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