| Autor: |
Ulloa-Stanojlović FM; School of Public Health, University of São Paulo, São Paulo, Brazil., Aguiar B; School of Public Health, University of São Paulo, São Paulo, Brazil., Jara LM; Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Peru., Sato MI; Companhia Ambiental do Estado de São Paulo-CETESB, São Paulo, Brazil., Guerrero JA; Servicio de Agua Potable y Alcantarillado de Lima-SEDAPAL, Lima, Peru., Hachich E; Companhia Ambiental do Estado de São Paulo-CETESB, São Paulo, Brazil., Matté GR; School of Public Health, University of São Paulo, São Paulo, Brazil., Dropa M; School of Public Health, University of São Paulo, São Paulo, Brazil., Matté MH; School of Public Health, University of São Paulo, São Paulo, Brazil. mhmatte@usp.br., de Araújo RS; School of Public Health, University of São Paulo, São Paulo, Brazil. |
| Abstrakt: |
The objectives of the study were to detect and genotype Cryptosporidium spp. and Giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the State of São Paulo, Brazil, and at the entrance of a Wastewater Treatment Plant (WWTP) in Lima, Peru. Samples were collected and concentrated by centrifugation. The genomic DNA was extracted for molecular characterization by nested PCR for Cryptosporidium and double nested PCR for Giardia, followed by sequencing and phylogenetic analysis. G. intestinalis was found in 63.6 % of the samples, and the human assemblages A and B were identified. Cryptosporidium sp. was found in 36.4 % of the samples, and the species were corresponding to Cryptosporidium hominis, Cryptosporidium cuniculus, and Cryptosporidium muris. Results revealed the presence of human pathogenic Cryptosporidium species and G. intestinalis human pathogenic assemblages. Molecular tools highlight the importance to map the genetic diversity of these parasites, as well as to detect their epidemiological circulation pathway in the environment. |
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