Detoxification of polycyclic aromatic hydrocarbons (PAHs) in Arabidopsis thaliana involves a putative flavonol synthase.

Autor: Hernández-Vega JC; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Cady B; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Kayanja G; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Mauriello A; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Cervantes N; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Gillespie A; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Lavia L; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Trujillo J; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA., Alkio M; Calvinstr. 25, D-24114 Kiel, Germany., Colón-Carmona A; Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA. Electronic address: adan.colon-carmona@umb.edu.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2017 Jan 05; Vol. 321, pp. 268-280. Date of Electronic Publication: 2016 Aug 24.
DOI: 10.1016/j.jhazmat.2016.08.058
Abstrakt: Polycyclic aromatic hydrocarbons (PAHs) are environmental contaminants with cytotoxic, teratogenic and carcinogenic properties. Bioremediation studies with bacteria have led to the identification of dioxygenases (DOXs) in the first step to degrade these recalcitrant compounds. In this study, we characterized the role of the Arabidopsis thaliana AT5G05600, a putative DOX of the flavonol synthase family, in the transformation of PAHs. Phenotypic analysis of loss-of-function mutant lines showed that these plant lines were less sensitive to the toxic effects of phenanthrene, suggesting possible roles of this gene in PAH degradation in vivo. Interestingly, these mutant lines showed less accumulation of H 2 O 2 after PAH exposure. Transgenic lines over-expressing At5g05600 showed a hypersensitive response and more oxidative stress after phenanthrene treatments. Moreover, fluorescence spectra results of biochemical assays with the recombinant His-tagged protein AT5G05600 detected chemical modifications of phenanthrene. Taken together, these results support the hypothesis that AT5G05600 is involved in the catabolism of PAHs and the accumulation of toxic intermediates during PAH biotransformation in plants. This research represents the first step in the design of transgenic plants with the potential to degrade PAHs, leading to the development of vigorous plant varieties that can reduce the levels of these pollutants in the environment.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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