Photothermal therapeutic effect of PEGylated gold nano-semicubes in chemically-induced skin cancer in mice.

Autor: Abo-Elfadl MT; Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt; Department of Biochemistry, National Research Centre, Cairo, Egypt., Gamal-Eldeen AM; Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt; Department of Biochemistry, National Research Centre, Cairo, Egypt. Electronic address: aeldeen7@yahoo.com., Elshafey MM; Biochemistry Department, Faculty of Pharmacy, Al Azhar University, Cairo, Egypt., Abdalla GM; Biochemistry Department, Faculty of Pharmacy, Al Azhar University, Cairo, Egypt., Ali SS; Biochemistry Department, Faculty of Pharmacy, Al Azhar University, Cairo, Egypt., Ali MR; Advanced Material Sciences and Nanotechnology Laboratory, Center of Excellence for Advanced Sciences, National Research Centre, Dokki 12622, Cairo, Egypt., Zawrah MF; Advanced Material Sciences and Nanotechnology Laboratory, Center of Excellence for Advanced Sciences, National Research Centre, Dokki 12622, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2016 Nov; Vol. 164, pp. 21-29. Date of Electronic Publication: 2016 Sep 13.
DOI: 10.1016/j.jphotobiol.2016.09.012
Abstrakt: Background: The photothermal properties of gold nanoparticles (GNPs) are promising therapeutic modality for cancer. The study objective is to evaluate the therapeutic effect of the prepared PEGylated gold nano-semicubes (PEG-GNSCs) in skin cancer. The synthesized PEG-GNSCs were intermediate between cubic and rod shapes (low aspect ratio- rods).
Methods: In vitro toxicity was investigated in human skin melanoma Sk-Mel-28 cells, and skin squamous cell carcinoma was induced in CD1 mice by dimethylbenzanthracene (DMBA) and 12-O-tetradecanoyl-phorbol-13-acetate (TPA).
Results: The calculated IC 50 in Sk-Mel-28 cells was 3.41μg/ml of PEG-GNSCs, in presence of laser exposure. Photothermal therapy using laser-stimulated PEG-GNSCs resulted in inhibited volume of skin tumors. Our findings indicated that the inflammatory mediators, nitric oxide and cycloxygenase-2, were inhibited in mice after being treated with low and high doses of PEG-GNSCs, accompanied with laser exposure. However, the tumor necrosis factor -α was markedly elevated, while there was no change in 5-lipoxygenase. The pro-angiogenic factor vascular endothelial growth factor was inhibited, while histone acetylation and apoptosis were induced in tumor-bearing groups, after being treated with laser-stimulated PEG-GNSCs.
Conclusion: The present study indicated the promising photothermal therapeutic effect of laser-stimulated PEG-GNSCs as an effective modality to inhibit the tumor growth, the angiogenesis and partially the inflammation.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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