Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology.

Autor:	Mendes NJ; Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil., Cruz VP; Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil., Ashikaga FY; Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil., Camargo SM; Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil; Laboratório de Genética Pesqueira e Conservação, Institute of Marine Sciences, Universidade Federal de São Paulo, Santos, São Paulo, Brazil., Oliveira C; Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil., Piercy AN; Lake Nona Campus, Valencia College , Orlando, Florida , United States., Burgess GH; Florida Museum of Natural History, Florida Program for Shark Research, University of Florida , Gainesville, Florida , United States., Coelho R; IPMA, Portuguese Institute for the Ocean and Atmosphere , Algarve , Portugal., Santos MN; IPMA, Portuguese Institute for the Ocean and Atmosphere , Algarve , Portugal., Mendonça FF; Laboratório de Genética Pesqueira e Conservação, Institute of Marine Sciences, Universidade Federal de São Paulo , Santos, São Paulo , Brazil., Foresti F; Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2016 Aug 30; Vol. 4, pp. e2205. Date of Electronic Publication: 2016 Aug 30 (Print Publication: 2016).
DOI:	10.7717/peerj.2205
Abstrakt:	The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide. Competing Interests: The authors declare that they have no competing interests.
Databáze:	MEDLINE
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