Cryopreservation of Indian red jungle fowl (Gallus gallus murghi) semen.
Autor: | Rakha BA; Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan. Electronic address: arbushra@uaar.edu.pk., Ansari MS; Department of Zoology, University of Sargodha, Lyallpur Campus, Faisalabad 38000, Pakistan., Akhter S; Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan., Hussain I; Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan., Blesbois E; INRA, UMR-85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, 37380, Nouzilly, France. |
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Jazyk: | angličtina |
Zdroj: | Animal reproduction science [Anim Reprod Sci] 2016 Nov; Vol. 174, pp. 45-55. Date of Electronic Publication: 2016 Sep 06. |
DOI: | 10.1016/j.anireprosci.2016.09.004 |
Abstrakt: | The population of red jungle fowl is declining and needs special attention for its conservation with suitable approaches. For ex situ in vitro conservation of Indian red jungle fowl, establishment of semen cryobank is an appropriate option, for which an extender with adequate retrieval capacity for functional spermatozoa is required. Therefore, studies were designed to evaluate a wide range of extenders for cryopreservation of Indian red jungle fowl (Gallus gallus murghi) sperm to achieve maximal post-thawed semen quality and fertility. For this purpose, semen from eight mature cocks were collected, initially evaluated (percent sperm motility, volume and concentration), pooled, assessed for motility, plasma membrane integrity, viability and acrosome integrity, and divided into six aliquots for dilution (1:5; 37°C) in Beltsville poultry, red fowl extender, Lake, EK, Tselutin poultry and chicken semen extenders. Diluted semen was cooled from 37°C to 4°C @ -0.275°C/min. Glycerol (20%) was added to chilled semen, equilibrated for 10min, filled in 0.5mL French straws, kept over LN (Copyright © 2016 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
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