Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain.

Autor:	Pupulin ÁRT; Laboratory of Parasitology, Department of Basic Sciences of Health, State University of Maringá, Paraná, Brazil., Bracht L; Laboratory of Hepatic Metabolism, Department of Biochemistry, State University of Maringá, Paraná, Brazil., de Oliveira Dalalio MM; Laboratory of Immunology, Department of Basic Sciences of Health, State University of Maringá, Paraná, Brazil., de Souza Silva-Comar FM; Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil., da Rocha BA; Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil., Ames FQ; Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil., Cuman RKN; Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil., Bersani-Amado CA; Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil. Electronic address: ciomar.amado@gmail.com.
Jazyk:	angličtina
Zdroj:	Asian Pacific journal of tropical medicine [Asian Pac J Trop Med] 2016 Sep; Vol. 9 (9), pp. 860-865. Date of Electronic Publication: 2016 Jul 26.
DOI:	10.1016/j.apjtm.2016.07.007
Abstrakt:	Objective: To identify whether Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain. Methods: Animals were divided into five groups: non-treated infected animals (I); benznidazole-treated infected animals (Bz; 100 mg/kg body weight, single daily dose by gavage); Canova medication (CM) treated infected animals (CM; 0.2 mL/animal, single daily dose by gavage); benznidazole- and Canova medication-treated infected animals with the above-mentioned dose (Bz+CM); and non-infected animals (C). TNF-α and IL-10 levels were determined in serum aliquots after 4, 7, 10, 13, and 29 days of infection. An ELISA technique was employed with R&D System Inc. antibody pairs. Results: A high increase in TNF-α and IL-10 levels occurred in the infected and CM-treated groups within the treatment employed on the 10th day after infection, coupled with a IL-10 decrease on the 13th day after infection when compared with the other experimental groups. Conclusions: CM may change the balance between plasma cytokine levels (TNF-α and IL-10) in mice infected with Y strain T. cruzi, with important consequences leading towards a more severe infection. (Copyright © 2016 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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