Induction of Robust Immune Responses in Swine by Using a Cocktail of Adenovirus-Vectored African Swine Fever Virus Antigens.

Autor:	Lokhandwala S; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA SLokhandwala@cvm.tamu.edu WMwangi@cvm.tamu.edu., Waghela SD; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA., Bray J; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA., Martin CL; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA., Sangewar N; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA., Charendoff C; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA., Shetti R; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA., Ashley C; Department of Large Animal Clinical Sciences, Texas A&M University, College Station, Texas, USA., Chen CH; Department of Poultry Science, Texas A&M University, College Station, Texas, USA., Berghman LR; Department of Poultry Science, Texas A&M University, College Station, Texas, USA., Mwangi D; Zoetis, Kalamazoo, Michigan, USA., Dominowski PJ; Zoetis, Kalamazoo, Michigan, USA., Foss DL; Zoetis, Kalamazoo, Michigan, USA., Rai S; Zoetis, Kalamazoo, Michigan, USA., Vora S; Zoetis, Kalamazoo, Michigan, USA., Gabbert L; Plum Island Animal Disease Center, U.S. Department of Homeland Security Science and Technology Directorate, Greenport, New York, USA., Burrage TG; Plum Island Animal Disease Center, U.S. Department of Homeland Security Science and Technology Directorate, Greenport, New York, USA., Brake D; Plum Island Animal Disease Center, U.S. Department of Homeland Security Science and Technology Directorate, Greenport, New York, USA., Neilan J; Plum Island Animal Disease Center, U.S. Department of Homeland Security Science and Technology Directorate, Greenport, New York, USA., Mwangi W; Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, USA SLokhandwala@cvm.tamu.edu WMwangi@cvm.tamu.edu.
Jazyk:	angličtina
Zdroj:	Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2016 Nov 04; Vol. 23 (11), pp. 888-900. Date of Electronic Publication: 2016 Nov 04 (Print Publication: 2016).
DOI:	10.1128/CVI.00395-16
Abstrakt:	The African swine fever virus (ASFV) causes a fatal hemorrhagic disease in domestic swine, and at present no treatment or vaccine is available. Natural and gene-deleted, live attenuated strains protect against closely related virulent strains; however, they are yet to be deployed and evaluated in the field to rule out chronic persistence and a potential for reversion to virulence. Previous studies suggest that antibodies play a role in protection, but induction of cytotoxic T lymphocytes (CTLs) could be the key to complete protection. Hence, generation of an efficacious subunit vaccine depends on identification of CTL targets along with a suitable delivery method that will elicit effector CTLs capable of eliminating ASFV-infected host cells and confer long-term protection. To this end, we evaluated the safety and immunogenicity of an adenovirus-vectored ASFV (Ad-ASFV) multiantigen cocktail formulated in two different adjuvants and at two immunizing doses in swine. Immunization with the cocktail rapidly induced unprecedented ASFV antigen-specific antibody and cellular immune responses against all of the antigens. The robust antibody responses underwent rapid isotype switching within 1 week postpriming, steadily increased over a 2-month period, and underwent rapid recall upon boost. Importantly, the primed antibodies strongly recognized the parental ASFV (Georgia 2007/1) by indirect fluorescence antibody (IFA) assay and Western blotting. Significant antigen-specific gamma interferon-positive (IFN-γ + ) responses were detected postpriming and postboosting. Furthermore, this study is the first to demonstrate induction of ASFV antigen-specific CTL responses in commercial swine using Ad-ASFV multiantigens. The relevance of the induced immune responses in regard to protection needs to be evaluated in a challenge study. (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
Databáze:	MEDLINE
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