A Protocol to Enhance INS1E and MIN6 Functionality-The Use of Theophylline.
| Autor: | Groot Nibbelink M; Developmental BioEngineering, MIRA Institute of Biomedical Technology and Technical Medicine, University of Twente, Enschede 7522 NB, The Netherlands. m.grootnibbelink@utwente.nl., Marchioli G; Developmental BioEngineering, MIRA Institute of Biomedical Technology and Technical Medicine, University of Twente, Enschede 7522 NB, The Netherlands. g.marchioli@tue.nl., Moroni L; Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht 6200 MD, The Netherlands. l.moroni@maastrichtuniversity.nl., Karperien M; Developmental BioEngineering, MIRA Institute of Biomedical Technology and Technical Medicine, University of Twente, Enschede 7522 NB, The Netherlands. h.b.j.karperien@utwente.nl., Van Apeldoorn A; Developmental BioEngineering, MIRA Institute of Biomedical Technology and Technical Medicine, University of Twente, Enschede 7522 NB, The Netherlands. a.a.vanapeldoorn@utwente.nl. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of molecular sciences [Int J Mol Sci] 2016 Sep 12; Vol. 17 (9). Date of Electronic Publication: 2016 Sep 12. |
| DOI: | 10.3390/ijms17091532 |
| Abstrakt: | In vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model for islets of Langerhans for in vitro research. The effect of theophylline is dose- and cell line-dependent, resulting in a minimal stimulation index of five followed by a rapid return to baseline insulin secretion by reducing glucose concentrations after a first high glucose stimulation. This protocol solves issues concerning in vitro research for type I diabetes as donors and the availability of primary islets of Langerhans are limited. To avoid the limitations of using human donor material, cell lines represent a valid alternative. Many different β cell lines have been reported, but the lack of reproducible responsiveness to glucose stimulation remains a challenge. Competing Interests: The authors declare no conflict of interest. |
| Databáze: | MEDLINE |
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