Variation in the Dicer and RAN Genes Are Associated with Survival in Patients with Hepatocellular Carcinoma.

Autor: Kim MN; Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, South Korea., Kim JO; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea., Lee SM; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea., Park H; Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, South Korea., Lee JH; Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, South Korea., Rim KS; Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, South Korea., Hwang SG; Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, South Korea., Kim NK; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2016 Sep 09; Vol. 11 (9), pp. e0162279. Date of Electronic Publication: 2016 Sep 09 (Print Publication: 2016).
DOI: 10.1371/journal.pone.0162279
Abstrakt: Single-nucleotide polymorphisms (SNPs) in microRNA machinery genes might affect microRNA processing and subsequently impact tumorigenesis. The aim of this study was to investigate the associations between SNPs in microRNA machinery genes and hepatocellular carcinoma (HCC) in a Korean population. Genotyping of six SNPs in microRNA machinery genes was performed using blood samples from 147 patients with HCC and 209 healthy control subjects. None of the six SNPs in microRNA machinery genes were significantly associated with HCC development. However, among the models for six polymorphic loci-DICER (rs3742330 and rs13078), DROSHA (rs10719 and rs6877842), RAN (rs14035) and XPO5 (rs11077)-one allele combination (A-A-T-C-C-C) showed synergistic effects in terms of an increased risk of HCC development (odds ratio = 8.881, 95% confidence interval [CI] = 1.889-41.750; P = 0.002). Multivariate Cox proportional hazard regression analysis showed a significant survival benefit for the DICER rs3742330 GG compared with the AA type (hazard ratio [HR], 0.314; 95% CI, 0.135-0.730; P = 0.007) and for the RAN rs14035 CT compared with the CC genotype (HR, 0.587; 95% CI, 0.349-0.987; P = 0.044). Although we found no direct association between DICER (rs3742330 and rs13078), DROSHA (rs10719 and rs6877842), RAN (rs14035) or XPO5 (rs11077) polymorphisms and HCC risk, we demonstrated that DICER (rs3742330) and RAN (rs14035) were associated with the survival of HCC patients. Future studies with larger samples are needed to determine associations of SNPs in microRNA machinery genes with HCC risk and prognosis.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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