Yersinia pestis Caf1 Protein: Effect of Sequence Polymorphism on Intrinsic Disorder Propensity, Serological Cross-Reactivity and Cross-Protectivity of Isoforms.

Autor: Kopylov PKh; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia., Platonov ME; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia., Ablamunits VG; Saint Petersburg Medical Pediatric University, Saint Petersburg, Russia., Kombarova TI; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia., Ivanov SA; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia., Kadnikova LA; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia., Somov AN; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia., Dentovskaya SV; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia., Uversky VN; Department of Molecular Medicine and Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, Florida, United States of America.; Laboratory of New Methods in Biology, Institute for Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region, Russia., Anisimov AP; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2016 Sep 08; Vol. 11 (9), pp. e0162308. Date of Electronic Publication: 2016 Sep 08 (Print Publication: 2016).
DOI: 10.1371/journal.pone.0162308
Abstrakt: Yersinia pestis Caf1 is a multifunctional protein responsible for antiphagocytic activity and is a key protective antigen. It is generally conserved between globally distributed Y. pestis strains, but Y. pestis subsp. microtus biovar caucasica strains circulating within populations of common voles in Georgia and Armenia were reported to carry a single substitution of alanine to serine. We investigated polymorphism of the Caf1 sequences among other Y. pestis subsp. microtus strains, which have a limited virulence in guinea pigs and in humans. Sequencing of caf1 genes from 119 Y. pestis strains belonging to different biovars within subsp. microtus showed that the Caf1 proteins exist in three isoforms, the global type Caf1NT1 (Ala48 Phe117), type Caf1NT2 (Ser48 Phe117) found in Transcaucasian-highland and Pre-Araks natural plague foci #4-7, and a novel Caf1NT3 type (Ala48 Val117) endemic in Dagestan-highland natural plague focus #39. Both minor types are the progenies of the global isoform. In this report, Caf1 polymorphism was analyzed by comparing predicted intrinsic disorder propensities and potential protein-protein interactivities of the three Caf1 isoforms. The analysis revealed that these properties of Caf1 protein are minimally affected by its polymorphism. All protein isoforms could be equally detected by an immunochromatography test for plague at the lowest protein concentration tested (1.0 ng/mL), which is the detection limit. When compared to the classic Caf1NT1 isoform, the endemic Caf1NT2 or Caf1NT3 had lower immunoreactivity in ELISA and lower indices of self- and cross-protection. Despite a visible reduction in cross-protection between all Caf1 isoforms, our data suggest that polymorphism in the caf1 gene may not allow the carriers of Caf1NT2 or Caf1NT3 variants escaping from the Caf1NT1-mediated immunity to plague in the case of a low-dose flea-borne infection.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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