| Autor: |
Ramos-Martínez E; Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional 'Siglo XXI', Instituto Mexicano del Seguro Social (IMSS)., Lascurain R, Tenorio EP, Sánchez-González A, Chávez-Rueda K, Chávez-Sánchez L, Jara-Quezada LJ, Chávez-Sánchez R, Zenteno E, Blanco-Favela F |
| Jazyk: |
angličtina |
| Zdroj: |
The Tohoku journal of experimental medicine [Tohoku J Exp Med] 2016 Sep; Vol. 240 (1), pp. 79-89. |
| DOI: |
10.1620/tjem.240.79 |
| Abstrakt: |
T cells from patients with systemic lupus erythematosus (SLE) show a decreased activation threshold and increased apoptosis. These processes seem to be regulated by glycosylated molecules on the T cell surface. Here, we determined through flow cytometry the expression of mucin-type O-glycans on T helper cells in peripheral blood mononuclear cells (PBMC) from 23 SLE patients and its relation with disease activity. We used lectins specific for the disaccharide Gal-GalNAc, such as Amaranthus leucocarpus lectin (ALL), Artocarpus integrifolia lectin (jacalin) and Arachis hypogaea lectin (peanut agglutinin, PNA), as well as lectins for sialic acid such as Sambucus nigra agglutinin (SNA) and Maakia amurensis agglutinin (MAA). The results showed that ALL, but not jacalin or PNA, identified significant differences in O-glycan expression on T helper cells from active SLE patients (n = 10). Moreover, an inverse correlation was found between the frequency of T helper cells recognized by ALL and SLE Disease Activity Index (SLEDAI) score in SLE patients. In contrast, SNA and MAA lectins did not identify any differences between CD4(+) T cells from SLE patients. There was no difference in the recognition by ALL on activated T helper cells and T regulatory (Treg) cells. Our findings point out that activation of SLE disease diminishes the expression of O-glycans in T helper cells; ALL could be considered as a marker to determine activity of the disease. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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