Whole genome SNP scanning of snow sheep (Ovis nivicola).

Autor:	Deniskova TE; Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia. horarka@yandex.ru., Okhlopkov IM; Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.; Institute of Biological Problems of Cryolithozone, Siberian Branch, Russian Academy of Sciences, ul. Lenina 41, Yakutsk, 677980, Russia., Sermyagin AA; Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia., Gladyr' EA; Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia., Bagirov VA; Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia., Sölkner J; Division of Livestock Sciences, University of Natural Resources and Life Sciences, Augasse 2-6, Vienna, A-1090, Austria., Mamaev NV; Institute of Biological Problems of Cryolithozone, Siberian Branch, Russian Academy of Sciences, ul. Lenina 41, Yakutsk, 677980, Russia., Brem G; Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.; Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinärplatz, Vienna, A-1210, Austria., Zinov'eva NA; Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.
Jazyk:	angličtina
Zdroj:	Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2016 Jul; Vol. 469 (1), pp. 288-93. Date of Electronic Publication: 2016 Sep 07.
DOI:	10.1134/S1607672916040141
Abstrakt:	This is the first report performing the whole genome SNP scanning of snow sheep (Ovis nivicola). Samples of snow sheep (n = 18) collected in six different regions of the Republic of Sakha (Yakutia) from 64° to 71° N. For SNP genotyping, we applied Ovine 50K SNP BeadChip (Illumina, United States), designed for domestic sheep. The total number of genotyped SNPs (call rate 90%) was 47796 (88.1% of total SNPs), wherein 1006 SNPs were polymorphic (2.1%). Principal component analysis (PCA) showed the clear differentiation within the species O. nivicola: studied individuals were distributed among five distinct arrays corresponding to the geographical locations of sampling points. Our results demonstrate that the DNA chip designed for domestic sheep can be successfully used to study the allele pool and the genetic structure of snow sheep populations.
Databáze:	MEDLINE
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