Evaluation of acute toxicity, genotoxicity and inhibitory effect on acute inflammation of an ethanol extract of Morus alba L. (Moraceae) in mice.

Autor: Oliveira AM; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil., Nascimento MF; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil., Ferreira MR; Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, 50740-520 Recife, Pernambuco, Brazil., Moura DF; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, 55604-000 Vitória de Santo Antão, Pernambuco, Brazil., Souza TG; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, 55604-000 Vitória de Santo Antão, Pernambuco, Brazil., Silva GC; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil., Ramos EH; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil., Paiva PM; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil., Medeiros PL; Departamento de Histologia e Embriologia, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Pernambuco, Brazil., Silva TG; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil., Soares LA; Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, 50740-520 Recife, Pernambuco, Brazil., Chagas CA; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, 55604-000 Vitória de Santo Antão, Pernambuco, Brazil., Souza IA; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil., Napoleão TH; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil. Electronic address: thiagohn86@yahoo.com.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2016 Dec 24; Vol. 194, pp. 162-168. Date of Electronic Publication: 2016 Sep 03.
DOI: 10.1016/j.jep.2016.09.004
Abstrakt: Ethnopharmacological Relevance: Morus alba L. (white mulberry) is used in traditional medicine worldwide, including Brazil. The leaves of this plant are used to treat inflammatory disorders. Universal interest in this plant necessitates studies on the toxicological safety and scientific substantiation of the medicinal properties of M. alba. In previous work, we investigated the acute toxicity of orally administered M. alba ethanol extract in mice.
Aim of the Study: This work was designed to investigate the ethanol extract obtained from M. alba leaves for acute toxicity when intraperitoneally administered, in vivo genotoxicity, and potential to reduce acute inflammation. In order to further investigate the constituents of the extract, we also obtained the high-performance liquid chromatography (HPLC) fingerprint of the extract.
Materials and Methods: Phytochemical analysis by thin layer chromatography (TLC) was performed and the results were used to obtain the HPLC fingerprint. Acute toxicity of 300 and 2000mg/kg b.w. i.p. doses administered to mice for 14 days was evaluated. Genotoxicity was evaluated by counting the number of micronucleated polychromatic erythrocytes in the blood of mice that either received or did not receive the extract at 75, 150 and 300mg/kg b.w. per os. The anti-inflammatory effect of the same doses administered per os was investigated using the carrageenan air pouch model.
Results: The TLC analysis of the extract revealed the presence of a remarkable amount of flavonoids and cinnamic acids. The HPLC fingerprint showed the presence of one major peak corresponding to chlorogenic acid and two smaller peaks corresponding to flavonoids. In the toxicity assays, there were no deaths or deviations in behavior of treated mice as compared to the control at any dose. However, biochemical, hematological, and histological analyses showed that intraperitoneal injection caused several forms of damage to the mice, which were not observed in case of oral administration, studied in our previous work. Oral administration of the extract did not result in genotoxicity and considerably reduced (58.6-65.6% inhibition) leukocyte migration in all doses evaluated, in comparison with the negative control.
Conclusions: The ethanol extract from M. alba leaves administered intraperitoneally possesses a greater degree of toxicity in mice when compared to per os administration. The extract was not genotoxic when ingested by mice and exhibited a highly inhibitory effect against acute inflammation, which is probably linked to the presence of chlorogenic acid and flavonoids in the composition. This work contributes to the determination of safety of the medicinal use of M. alba leaves.
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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