Decoding the Long Noncoding RNA During Cardiac Maturation: A Roadmap for Functional Discovery.

Autor:	Touma M; The Children's Discovery and Innovation Institute (CDI), Department of Pediatrics, University of California, Los Angeles, CA.; Cardiovascular Research Laboratory, University of California, Los Angeles, CA., Kang X; The Children's Discovery and Innovation Institute (CDI), Department of Pediatrics, University of California, Los Angeles, CA., Zhao Y; The Children's Discovery and Innovation Institute (CDI), Department of Pediatrics, University of California, Los Angeles, CA., Cass AA; Department of Integrative Biology and Physiology, University of California, Los Angeles, CA., Gao F; Department of Neurology and Psychiatry, University of California, Los Angeles, CA., Biniwale R; Department of Cardiothoracic Surgery, University of California, Los Angeles, CA., Coppola G; Department of Neurology and Psychiatry, University of California, Los Angeles, CA., Xiao X; Department of Integrative Biology and Physiology, University of California, Los Angeles, CA., Reemtsen B; Department of Cardiothoracic Surgery, University of California, Los Angeles, CA., Wang Y; Cardiovascular Research Laboratory, University of California, Los Angeles, CA.; Department of Anesthesiology, Physiology and Medicine, University of California, Los Angeles, CA.
Jazyk:	angličtina
Zdroj:	Circulation. Cardiovascular genetics [Circ Cardiovasc Genet] 2016 Oct; Vol. 9 (5), pp. 395-407. Date of Electronic Publication: 2016 Sep 02.
DOI:	10.1161/CIRCGENETICS.115.001363
Abstrakt:	Background: Cardiac maturation during perinatal transition of heart is critical for functional adaptation to hemodynamic load and nutrient environment. Perturbation in this process has major implications in congenital heart defects. Transcriptome programming during perinatal stages is an important information but incomplete in current literature, particularly, the expression profiles of the long noncoding RNAs (lncRNAs) are not fully elucidated. Methods and Results: From comprehensive analysis of transcriptomes derived from neonatal mouse heart left and right ventricles, a total of 45 167 unique transcripts were identified, including 21 916 known and 2033 novel lncRNAs. Among these lncRNAs, 196 exhibited significant dynamic regulation along maturation process. By implementing parallel weighted gene co-expression network analysis of mRNA and lncRNA data sets, several lncRNA modules coordinately expressed in a developmental manner similar to protein coding genes, while few lncRNAs revealed chamber-specific patterns. Out of 2262 lncRNAs located within 50 kb of protein coding genes, 5% significantly correlate with the expression of their neighboring genes. The impact of Ppp1r1b-lncRNA on the corresponding partner gene Tcap was validated in cultured myoblasts. This concordant regulation was also conserved in human infantile hearts. Furthermore, the Ppp1r1b-lncRNA/Tcap expression ratio was identified as a molecular signature that differentiated congenital heart defect phenotypes. Conclusions: The study provides the first high-resolution landscape on neonatal cardiac lncRNAs and reveals their potential interaction with mRNA transcriptome during cardiac maturation. Ppp1r1b-lncRNA was identified as a regulator of Tcap expression, with dynamic interaction in postnatal cardiac development and congenital heart defects. (© 2016 American Heart Association, Inc.)
Databáze:	MEDLINE
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