N-3 long chain polyunsaturated fatty acids increase the expression of PPARγ-target genes and resistance of isolated heart and cultured cardiomyocytes to ischemic injury.

Autor: Shysh AM; Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev, Ukraine. Electronic address: angela@biph.kiev.ua., Nagibin VS; Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev, Ukraine. Electronic address: nagibin@biph.kiev.ua., Kaplinskii SP; Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev, Ukraine. Electronic address: Serg-kaplinskiy@bigmir.net., Dosenko VE; Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev, Ukraine. Electronic address: dosenko@biph.kiev.ua.
Jazyk: angličtina
Zdroj: Pharmacological reports : PR [Pharmacol Rep] 2016 Dec; Vol. 68 (6), pp. 1133-1139. Date of Electronic Publication: 2016 Aug 31.
DOI: 10.1016/j.pharep.2016.06.013
Abstrakt: Background: The goal of present investigation is to check the hypothesis that cardioprotective effect of polyunsaturated fatty acids (PUFAs) is mediated by influence on mRNA expression level of the FATP, IL-1ra and GJP43 through stimulation of PPARγ.
Methods: Animals obtained n-3 PUFAs orally during 4 weeks (0.1ml/100g b.w. per day) or during prenatal period. In experiments, isolated perfused hearts were subjected to 20-min regional ischemia and 40min reperfusion. The hearts of newborn rats (2-3days old) were used for isolated cardiomyocytes culture preparing. Culture cells underwent 30min of anoxia followed by 60min of reoxygenation. Using rtPCR the level of FATP, IL-1ra, GJP43 and BCL2 mRNA in isolated cardiomyocyte and hearts was evaluated.
Results: The data obtained indicate that in heart tissues from pups with prenatal n-3 PUFAs application the level of LA and DHA acids were increased in 3.6-fold and 2-fold correspondingly comparing to control. In adult hearts the level of DHA was increased in 1.4-fold, and the level of EPA-in 6.9-fold. We observed the increase in mRNA level of PPARγ-target genes: FATP in 2.25 times, and IL-1ra in 8.4 times. At the same time the level of mRNA of antiapoptotic gene BCL2 was increased in 2.13 times and Connexin-43 gene in 2.2 times after n-3 PUFAs application. These effects were accompanied by significantly improved cardiac function, and increase of living cardiomyocytes number at modeling of ischemia-reperfusion.
Conclusions: n-3 PUFAs application has cardioprotective effects and increases mRNA level of FATP, IL-1ra, GJP43, and BCL2 genes in culture of neonatal cardiomyocytes and in adult hearts.
(Copyright © 2016 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.)
Databáze: MEDLINE
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