Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins.

Autor: Fang Z; Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China.; Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China., Zhang S; Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China., Wang Y; Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China., Shen S; Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China., Wang F; Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China., Hao Y; Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China., Li Y; Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China., Zhang B; Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China., Zhou Y; Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China., Yang H; Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China. hy192@tom.com.
Jazyk: angličtina
Zdroj: BMC cancer [BMC Cancer] 2016 Sep 01; Vol. 16, pp. 706. Date of Electronic Publication: 2016 Sep 01.
DOI: 10.1186/s12885-016-2735-x
Abstrakt: Background: We previously described several abnormally expressed long non-coding RNA (lncRNA) in tong squamous cell carcinomas (TSCCs) that might be associated with tumor progression. In the present study, we aimed to investigate the role of abnormally expressed metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) lncRNA in the metastatic potential of TSCC cells and its molecular mechanisms.
Methods: Expression levels of MALAT-1 lncRNA were examined via quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in 127 TSCC samples as well as paired adjacent normal tissues and lymph node metastases (if exist). Lentiviral vectors expressing short hairpin RNA (shRNA) were used to knock down the expression of MALAT1 gene in two TSCC cell lines (CAL27 and SCC-25) with relatively higher MALAT-1 expression. Proliferational ability of the TSCC cells was analyzed using water soluble tetrazolium-1 (WST-1) assay. Metastatic abilities of TSCC cells were estimated in-vitro and in-vivo. We also performed a microarray-based screen to identify the genes influenced by MALAT-1 alteration, which were validated by real-time PCR analysis.
Results: Expression of MALAT-1 lncRNA was enhanced in TSCCs, especially in those with lymph node metastasis (LNM). Knockdown (KD) of MALAT-1 lncRNA in TSCC cells led to impaired migration and proliferation ability in-vitro and fewer metastases in-vivo. DNA microarray analysis showed that several members of small proline rich proteins (SPRR) were up-regulated by KD of MALAT-1 lncRNA in TSCC cells. SPRR2A over-expression could impair distant metastasis of TSCC cells in-vivo.
Conclusion: Enhanced expression of MALAT-1 is associated with the growth and metastatic potential of TSCCs. Knock down of MALAT-1 in TSCCs leads to the up-regulation of certain SPRR proteins, which influenced the distant metastasis of TSCC cells.
Databáze: MEDLINE
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