Histological response to platelet-rich plasma added to polypropylene mesh implemented in rabbits.

Autor:	Ávila OR; Departamento de Cirurgia, Universidade do Oeste Paulista, Presidente Prudente, São Paulo, Brasil., Parizzi NG; Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, Paraná, Brasil., Souza AP; Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, Paraná, Brasil., Botini DS; Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, Paraná, Brasil., Alves JY; Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, Paraná, Brasil., Almeida SH; Departamento de Cirurgia, Universidade Estadual de Londrina, Londrina, Paraná, Brasil.
Jazyk:	angličtina
Zdroj:	International braz j urol : official journal of the Brazilian Society of Urology [Int Braz J Urol] 2016 Sep-Oct; Vol. 42 (5), pp. 993-998.
DOI:	10.1590/S1677-5538.IBJU.2015.0319
Abstrakt:	Introduction: The platelet-rich plasma (PRP) is part of a set of biotechnologies, providing some growth factors that promote repair of different tissues. The polypropylene meshes (PPM) are applied in the correction of abdominal defects, pelvic floor and urinary incontinence, however, they induce many significant complications, as a result of an inappropriate inflammatory response. Purpose: To investigate the changes caused by PRP associated with the implantation of PPM in the abdomen of female rabbits, in the production of collagen I and III and the inflammatory infiltrate (ININ). Materials and Methods: We performed implant meshes with and without PRP in adult rabbits (n=30) and euthanasia at 7, 30 and 90 days. Two plates were prepared from each animal and analyzed in five different fields. The ININ was evaluated by quantification of inflammatory cells using hematoxylin-eosin and the collagen by Sirius red method. The results were analyzed applying the Wilcoxon, Kruskal-Wallis, Junckheere and Friedmann tests. Results: There was a significant difference in the number of inflammatory cells between the groups with and without PRP (p=0.01) at 90 days. There was increased production of collagen I, III and total with the use of PRP, at seven days. Conclusion: The PPM coating with PRP was associated with increased ININ at the implant area, and an increasing trend during the process of tissue repair. The PPM coated with PRP was related to increased concentration of collagen I, collagen III and the concentration of total collagen increased after seven days of implantation. Competing Interests: Conflicts of Interest: None declared. (Copyright® by the International Brazilian Journal of Urology.)
Databáze:	MEDLINE
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