Rapid lipid a structure determination via surface acoustic wave nebulization and hierarchical tandem mass spectrometry algorithm.

Autor: Yoon SH; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, 650W Baltimore St., Baltimore, MD, 21201, USA., Liang T; Department of Pharmaceutical Science, School of Pharmacy, University of Maryland, 20N Pine St., Baltimore, MD, 21201, USA., Schneider T; Department of Pharmaceutical Science, School of Pharmacy, University of Maryland, 20N Pine St., Baltimore, MD, 21201, USA., Oyler BL; Department of Pharmaceutical Science, School of Pharmacy, University of Maryland, 20N Pine St., Baltimore, MD, 21201, USA., Chandler CE; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, 650W Baltimore St., Baltimore, MD, 21201, USA., Ernst RK; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, 650W Baltimore St., Baltimore, MD, 21201, USA., Yen GS; Deurion LLC, 3518 Fremont Ave #503, Seattle, WA, 98103, USA., Huang Y; Deurion LLC, 3518 Fremont Ave #503, Seattle, WA, 98103, USA., Nilsson E; Deurion LLC, 3518 Fremont Ave #503, Seattle, WA, 98103, USA., Goodlett DR; Department of Pharmaceutical Science, School of Pharmacy, University of Maryland, 20N Pine St., Baltimore, MD, 21201, USA.; Deurion LLC, 3518 Fremont Ave #503, Seattle, WA, 98103, USA.
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2016 Dec 15; Vol. 30 (23), pp. 2555-2560.
DOI: 10.1002/rcm.7728
Abstrakt: Rationale: Surface acoustic wave nebulization (SAWN) is an easy to use sample transfer method for rapid mass spectrometric analysis. A new standing wave (SW) SAWN chip, with higher ionization efficiency than our previously reported design, is used for rapid analysis of lipids.
Methods: The crude, yet fast, Caroff protocol was used for lipid A extraction from Francisella novicida. SW-SAWN with a Waters Synapt G2S quadrupole time-of-flight (QTOF) mass spectrometer was used to generate lipid A ions. Quadrupole collision-induced dissociation (Q-CID) of lipid A at varying CID energies was used to approximate the ion trap MS n data required for our hierarchical tandem mass spectrometry (HiTMS) algorithm. Structural hypotheses can be obtained directly from the HiTMS algorithm to identify species-specific lipid A molecules.
Results: SW-SAWN successfully generated ions from lipid A extracted from Francisella novicida using the faster Caroff method. In addition, varying collision energies were used to generate tandem mass spectra similar to MS 3 and MS 4 spectra from an ion trap. The Q-CID spectra are compatible with our HiTMS algorithm and offer an improvement over lipid A tandem mass spectra acquired in an ion trap.
Conclusions: Combining SW-SAWN and Q-CID enabled more structural assignments than previously reported in half the time. The ease of generating spectra by SAWN tandem MS in combination with HiTMS interpretation offers high-throughput lipid A structural analysis and thereby rapid detection of pathogens based on lipid fingerprinting. Copyright © 2016 John Wiley & Sons, Ltd.
(Copyright © 2016 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
Externí odkaz: