Two spatially and temporally distinct Ca 2+ signals convey Arabidopsis thaliana responses to K + deficiency.

Autor: Behera S; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany., Long Y; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100193, China., Schmitz-Thom I; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany., Wang XP; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100193, China., Zhang C; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany., Li H; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100193, China., Steinhorst L; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany., Manishankar P; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany., Ren XL; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100193, China., Offenborn JN; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany., Wu WH; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100193, China., Kudla J; Institut für Biologie und Biotechnologie der Pflanzen, Universität Münster, Schlossplatz 7, Münster, 48149, Germany.; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100193, China., Wang Y; State Key Laboratory of Plant Physiology and Biochemistry (SKLPPB), College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing, 100193, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2017 Jan; Vol. 213 (2), pp. 739-750. Date of Electronic Publication: 2016 Aug 31.
DOI: 10.1111/nph.14145
Abstrakt: In plants, potassium (K + ) homeostasis is tightly regulated and established against a concentration gradient to the environment. Despite the identification of Ca 2+ -regulated kinases as modulators of K + channels, the immediate signaling and adaptation mechanisms of plants to low-K + conditions are only partially understood. To assess the occurrence and role of Ca 2+ signals in Arabidopsis thaliana roots, we employed ratiometric analyses of Ca 2+ dynamics in plants expressing the Ca 2+ reporter YC3.6 in combination with patch-clamp analyses of root cells and two-electrode voltage clamp (TEVC) analyses in Xenopus laevis oocytes. K + deficiency triggers two successive and distinct Ca 2+ signals in roots exhibiting spatial and temporal specificity. A transient primary Ca 2+ signature arose within 1 min in the postmeristematic stelar tissue of the elongation zone, while a secondary Ca 2+ response occurred after several hours as sustained Ca 2+ elevation in defined tissues of the elongation and root hair differentiation zones. Patch-clamp and TEVC analyses revealed Ca 2+ dependence of the activation of the K + channel AKT1 by the CBL1-CIPK23 Ca 2+ sensor-kinase complex. Together, these findings identify a critical role of cell group-specific Ca 2+ signaling in low K + responses and indicate an essential and direct role of Ca 2+ signals for AKT1 K + channel activation in roots.
(© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.)
Databáze: MEDLINE
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