| Autor: |
Liu ZH; College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China., Ding JJ; College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China., Yang QQ; College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China., Song HZ; College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China., Chen XT; School of Pharmacy, Anhui Medical University, Hefei 230038, PR China., Xu Y; College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China., Xiao GR; College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China., Wang HL; College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China. |
| Abstrakt: |
Bisphenol-A (BPA, 4, 4'-isopropylidene-2-diphenol), a synthetic xenoestrogen that widely used in the production of polycarbonate plastics, has been reported to impair hippocampal development and function. Our previous study has shown that BPA exposure impairs Sprague-Dawley (SD) male hippocampal dendritic spine outgrowth. In this study, the sex-effect of chronic BPA exposure on spatial memory in SD male and female rats and the related synaptic mechanism were further investigated. We found that chronic BPA exposure impaired spatial memory in both SD male and female rats, suggesting a dysfunction of hippocampus without gender-specific effect. Further investigation indicated that BPA exposure causes significant impairment of dendrite and spine structure, manifested as decreased dendritic complexity, dendritic spine density and percentage of mushroom shaped spines in hippocampal CA1 and dentate gyrus (DG) neurons. Furthermore, a significant reduction in Arc expression was detected upon BPA exposure. Strikingly, BPA exposure significantly increased the mIPSC amplitude without altering the mEPSC amplitude or frequency, accompanied by increased GABAARβ2/3 on postsynaptic membrane in cultured CA1 neurons. In summary, our study indicated that Arc, together with the increased surface GABAARβ2/3, contributed to BPA induced spatial memory deficits, providing a novel molecular basis for BPA achieved brain impairment. |
