Molecular characteristics of Clostridium difficile isolates from human and animals in the North Eastern region of India.

Autor: Hussain I; Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Science & A.H. (FVSc & AH), SKUAST-Kashmir, Srinagar, 190006, J&K, India. Electronic address: isfaqhussain@yahoo.com., Borah P; Department of Microbiology, College of Veterinary Science (CVSc), Assam Agricultural University (AAU), Khanapara, Guwahati, 22, Assam, India; Department of Animal Biotechnology, CVSc, AAU, Khanapara, Guwahati, 22, Assam, India., Sharma RK; Department of Microbiology, College of Veterinary Science (CVSc), Assam Agricultural University (AAU), Khanapara, Guwahati, 22, Assam, India., Rajkhowa S; National Research Centre on Pig, ICAR, Rani, Guwahati, 31, Assam, India., Rupnik M; National Laboratory for Health, Environment and Food, Maribor, Slovenia; University of Maribor, Faculty of Medicine, Maribor, Slovenia; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, Slovenia., Saikia DP; Department of Animal Biotechnology, CVSc, AAU, Khanapara, Guwahati, 22, Assam, India., Hasin D; Division of Veterinary Physiology, FVSc & AH, SKUAST-Kashmir, Srinagar, 190006, India., Hussain I; Department of Animal Biotechnology, CVSc, AAU, Khanapara, Guwahati, 22, Assam, India., Deka NK; Department of Animal Biotechnology, CVSc, AAU, Khanapara, Guwahati, 22, Assam, India., Barkalita LM; Department of Animal Biotechnology, CVSc, AAU, Khanapara, Guwahati, 22, Assam, India., Nishikawa Y; Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, 558-8585, Japan., Ramamurthy T; National Institute of Cholera and Enteric Disease, Beleghata, Kolkata, 700 010, India.
Jazyk: angličtina
Zdroj: Molecular and cellular probes [Mol Cell Probes] 2016 Oct; Vol. 30 (5), pp. 306-311. Date of Electronic Publication: 2016 Aug 26.
DOI: 10.1016/j.mcp.2016.08.010
Abstrakt: A total of 1034 samples were collected from different sources and C. difficile was isolated from 18 (9.04%) of 199 human, 9 (4.89%) of 184 cattle, 29 (12.44%) of 233 pig, and from 23 (13.94%) of 165 poultry samples. Variations were observed on the rate of isolation according to age and clinical conditions (diarrhoea). None of the samples from cow, sheep, goat, local chicken, and wild animals yielded any C. difficile. Out of those isolates, 8, 2, 19 and 6 isolates from human, cattle, pig and poultry, respectively were toxigenic. The toxigenic isolates carried both tcdA, and tcdB (A + B + ) and most of the human and the pig isolates were also positive for binary toxin genes (cdtA and cdtB). The A + B + isolates belonged to three different toxinotypes (0, VI and XXXIII). Human and pig A + B + isolates belonged to three (045, 126 and ACD 019) and four (046, 087, 126 and ACD 011) different ribotypes, respectively and the ribotypes of two cattle isolates were 014 and ACD 010. Six A + B + avian isolates belonged to six different ribotypes (014, 087, SLO 134, SLO 160, ACD 012, ACD 014). The non-toxigenic isolates from human, cattle, pig and poultry were grouped into 7, 4, 4 and 7 different ribotypes, respectively. PFGE analysis could not differentiate similar ribotypes/toxinotypes of toxigenic isolates. All the toxigenic isolates showed cytopathic effect on Vero and Hela cell monolayers at 1:100 dilutions of cell-free culture supernatants within 18-20 h of inoculation.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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