Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP(+)-induced neurotoxicity via JNK pathway.

Autor: Oh Y; Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea., Jeong K; Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea., Kim K; Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea., Lee YS; Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea., Jeong S; Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea., Kim SS; Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea., Yoon KS; Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea., Ha J; Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea., Kang I; Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea., Choe W; Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea. Electronic address: wchoe@khu.ac.kr.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2016 Sep 23; Vol. 478 (3), pp. 1396-402. Date of Electronic Publication: 2016 Aug 25.
DOI: 10.1016/j.bbrc.2016.08.135
Abstrakt: Parkinson's disease (PD) is the second most common neurodegenerative disorder of aging. PD involves a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyidine (MPTP) and its toxic metabolite 1-methyl-4-phenylpyridinium ion (MPP+) inhibit the complex I of the mitochondrial electron transport chain, and have been widely used to construct PD models. Cyclophilin B (CypB) is an endoplasmic reticulum protein that binds to cyclosporine A as a cyclophilin family member. CypB has peptidyl-prolyl cis-trans isomerase (PPIase) activity. We investigated the protective effects of overexpressed CypB on MPP+-induced neurocytotoxicity in SH-SY5Y human neuroblastoma cells. Overexpressed CypB decreased MPP(+)-induced oxidative stress through the modulation of antioxidant enzymes including manganese superoxide dismutase and catalase, and prevented neurocytotoxicity via mitogen-activated protein kinase, especially the c-Jun N-terminal kinase pathway. In addition, CypB inhibited the activation of MPP(+)-induced the pro-apoptotic molecules poly (ADP-ribose) polymerase, Bax, and Bcl-2, and attenuated MPP(+)-induced mitochondrial dysfunction. The data suggest that overexpressed CypB protects neuronal cells from MPP+-induced dopaminergic neuronal cell death.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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