| Autor: |
Javurek AB; Department of Bond Life Sciences Center, University of Missouri, 1201 E. Rollins Road, Columbia, MO 65211, USA., Spollen WG; Department of Bond Life Sciences Center, University of Missouri, 1201 E. Rollins Road, Columbia, MO 65211, USA., Johnson SA; Department of Bond Life Sciences Center, University of Missouri, 1201 E. Rollins Road, Columbia, MO 65211, USA., Bivens NJ; DNA Core Facility, University of Missouri, 1201 E. Rollins Road, Columbia, MO 65211, USA., Bromert KH; DNA Core Facility, University of Missouri, 1201 E. Rollins Road, Columbia, MO 65211, USA., Givan SA; Department of Bond Life Sciences Center, University of Missouri, 1201 E. Rollins Road, Columbia, MO 65211, USA., Rosenfeld CS; Department of Bond Life Sciences Center, University of Missouri, 1201 E. Rollins Road, Columbia, MO 65211, USA. |
| Abstrakt: |
Our prior work showed that a novel microbiome resides in the seminal vesicles of wild-type and oestrogen receptor α (Esr1) knock-out mice and is impacted by the presence of functional Esr1 genes. The seminal fluid microbiome (SFM) may influence the health and reproductive status of the male, along with that of his partner and offspring. A high-fat diet (HFD) alters metabolites and other factors within seminal fluid and might affect the SFM. Adult (~15 weeks old) male mice were placed for 4 weeks on a control or high-fat diet and seminal fluid and fecal samples were collected, bacterial DNA isolated and subjected to 16s rRNA sequencing. Corynebacterium spp. were elevated in the seminal fluid of HFD males; however, Acinetobacter johnsonii, Streptophyta, Ammoniphilus spp., Bacillus spp. and Propionibacterium acnes were increased in control males. Rikenellaceae was more abundant in the fecal samples from HFD males. However, Bacteroides ovatus and another Bacteroides species, Bilophila, Sutterella spp., Parabacteroides, Bifidobacterium longum, Akkermansia muciniphila and Desulfovibrio spp. were greater in control males. Thus, short-term consumption of a HFD influences the seminal fluid and fecal microbiomes, which may have important health consequence for males and developmental origins of health and disease effects in resulting offspring. |
