Evaluation of the performance of a point-of-care method for total and differential white blood cell count in clozapine users.
| Autor: | Bui HN; Atalmedial Medical Diagnostic Centers, Hoofddorp, The Netherlands., Bogers JP; Psychiatric Centre Rivierduinen, Oegstgeest, The Netherlands., Cohen D; Department of Community Mental Health, Mental Health Service North Holland North, Heerhugowaard, The Netherlands., Njo T; Saltro Diagnostic Center for Primary Care, Utrecht, The Netherlands., Herruer MH; Atalmedial Medical Diagnostic Centers, Hoofddorp, The Netherlands. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of laboratory hematology [Int J Lab Hematol] 2016 Dec; Vol. 38 (6), pp. 703-709. Date of Electronic Publication: 2016 Aug 27. |
| DOI: | 10.1111/ijlh.12561 |
| Abstrakt: | Introduction: We evaluated the performance of the HemoCue WBC DIFF, a point-of-care device for total and differential white cell count, primarily to test its suitability for the mandatory white blood cell monitoring in clozapine use. Method: Leukocyte count and 5-part differentiation was performed by the point-of-care device and by routine laboratory method in venous EDTA-blood samples from 20 clozapine users, 20 neutropenic patients, and 20 healthy volunteers. From the volunteers, also a capillary sample was drawn. Intra-assay reproducibility and drop-to-drop variation were tested. Results: The correlation between both methods in venous samples was r > 0.95 for leukocyte, neutrophil, and lymphocyte counts. The correlation between point-of-care (capillary sample) and routine (venous sample) methods for these cells was 0.772; 0.817 and 0.798, respectively. Only for leukocyte and neutrophil counts, the intra-assay reproducibility was sufficient. Conclusion: The point-of-care device can be used to screen for leukocyte and neutrophil counts. Because of the relatively high measurement uncertainty and poor correlation with venous samples, we recommend to repeat the measurement with a venous sample if cell counts are in the lower reference range. In case of clozapine therapy, neutropenia can probably be excluded if high neutrophil counts are found and patients can continue their therapy. (© 2016 John Wiley & Sons Ltd.) |
| Databáze: | MEDLINE |
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