High-throughput method for the quantitation of metabolites and co-factors from homocysteine-methionine cycle for nutritional status assessment.

Autor:	Da Silva L; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Collino S; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Cominetti O; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Martin FP; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Montoliu I; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Moreno SO; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Corthesy J; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Kaput J; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Kussmann M; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland., Monteiro JP; Department of Pediatrics, Faculty of Medicine, Nutrition & Metabolism, University of São Paulo, Bandeirantes Avenue, HCFMRP Campus USP, 3900, Ribeirão Preto, SP 14049-900, Brazil., Guiraud SP; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
Jazyk:	angličtina
Zdroj:	Bioanalysis [Bioanalysis] 2016 Sep; Vol. 8 (18), pp. 1937-49.
DOI:	10.4155/bio-2016-0112
Abstrakt:	Aim: There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover metabolites and co-factors. Results: We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility. Conclusion: This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.
Databáze:	MEDLINE
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