| Autor: |
Waller SB; Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil. waller.stefanie@yahoo.com.br., Madrid IM; Centro de Controle de Zoonoses (CCZ), Pelotas, RS, Brazil., Silva AL; Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil., Dias de Castro LL; Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil., Cleff MB; Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil., Ferraz V; Laboratório de Cromatografia, Instituto de Química, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Meireles MC; Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil., Zanette R; Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., de Mello JR; Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. |
| Abstrakt: |
This study evaluated the chemical, cytotoxic and anti-Sporothrix brasiliensis properties of commercial essential oils of rosemary (Rosmarinus officinalis L.), oregano (Origanum vulgare L.) and marjoram (Origanum majorana L.). Chemical composition of the oils was identified through gas chromatography with flame ionization detector, and cytotoxicity was performed through MTT assay in VERO cell line. Anti-S. brasiliensis activity was performed according to the CLSI M38-A2 guidelines using isolates obtained from cats and dogs. The major compounds found were carvacrol in the oregano oil (73.9 %) and 1,8-cineole in rosemary and marjoram oils (49.4 and 20.9 %, respectively). All S. brasiliensis isolates were susceptible to the plant oils, including itraconazole-resistant ones. Marjoram and rosemary oils showed MIC 90 of 0.56 and 1.12 mg ml -1 , and MFC 90 of 4.5 and 9 mg ml -1 , respectively. For oregano oil, a strong antifungal activity was observed with MIC 90 and MFC 90 values ≤0.07 mg ml -1 . The weakest cytotoxicity was observed for rosemary oil. Further studies should be undertaken to evaluate the safety and efficacy of these essential oils in sporotrichosis. |
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