Analyzing Synthetic Promoters Using Arabidopsis Protoplasts.
| Autor: | Stracke R; Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany., Thiedig K; Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany., Kuhlmann M; Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany., Weisshaar B; Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany. bernd.weisshaar@uni-bielefeld.de. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1482, pp. 67-81. |
| DOI: | 10.1007/978-1-4939-6396-6_5 |
| Abstrakt: | This chapter describes a transient protoplast co-transfection method that can be used to quantitatively study in vivo the activity and function of promoters and promoter elements (reporters), and their induction or repression by transcription factors (effectors), stresses, hormones, or metabolites. A detailed protocol for carrying out transient co-transfection assays with Arabidopsis At7 protoplasts and calculating the promoter activity is provided. |
| Databáze: | MEDLINE |
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