Evaluation of heterocyclic steroids and curcumin derivatives as anti-breast cancer agents: Studying the effect on apoptosis in MCF-7 breast cancer cells.

Autor: Elmegeed GA; Hormones Department, National Research Centre, Dokki, Giza, Egypt (ID:60014618). Electronic address: gamalae@hotmail.com., Yahya SM; Hormones Department, National Research Centre, Dokki, Giza, Egypt (ID:60014618)., Abd-Elhalim MM; Hormones Department, National Research Centre, Dokki, Giza, Egypt (ID:60014618)., Mohamed MS; Chemistry Department, Faculty of Science, Cairo University, Cairo, Egypt; Biochemistry Speciality, Faculty of Science, Cairo University, Cairo, Egypt., Mohareb RM; Chemistry Department, Faculty of Science, Cairo University, Cairo, Egypt., Elsayed GH; Hormones Department, National Research Centre, Dokki, Giza, Egypt (ID:60014618).
Jazyk: angličtina
Zdroj: Steroids [Steroids] 2016 Nov; Vol. 115, pp. 80-89. Date of Electronic Publication: 2016 Aug 21.
DOI: 10.1016/j.steroids.2016.08.014
Abstrakt: Anticancer agents consisting of hybrid molecules are used to improve effectiveness and diminish drug resistance. The current study aimed to introduce newly synthesized hetero-steroids of promising anticancer effects. Besides, the pro-apoptotic effects of new compounds were investigated extensively. Several pyrimidino-, triazolopyrimidino-, pyridazino-, and curcumin-steroid derivatives were synthesized, elucidated and confirmed using the spectral and analytical data. The synthesized hetero-steroids, compounds 9, 10, 11, 12, 13, 14, 15, 18, 20, 21, 22 and 24, were tested for their cytotoxic effects versus human breast cancer cells (MCF-7) using neutral red supravital dye uptake assay. Compound 24 (IC50=18μM) showed more inhibitory influence on MCF-7 growth. Using QRT-PCR (Quantitative real time-polymerase chain reaction), CCND1, Survivin, BCL-2, CDC2, P21 and P53, genes expression levels were investigated. The study results disclose that compounds 4, 7, 18, 24 knocked down the expression levels of CCND1, Survivin, BCL-2 and CDC2. However, P21 and P53 were up-regulated by compounds 21, 22. This study introduced promising pro-apoptotic anticancer agents acting through the modulation of key regulators of apoptosis and cell cycle genes.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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