An ( R )-Imine Reductase Biocatalyst for the Asymmetric Reduction of Cyclic Imines.

Autor: Hussain S; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester, M1 7DN (UK)., Leipold F; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester, M1 7DN (UK)., Man H; Department of Chemistry, University of York, Heslington, York, YO10 5DD (UK)., Wells E; Department of Chemistry, University of York, Heslington, York, YO10 5DD (UK)., France SP; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester, M1 7DN (UK)., Mulholland KR; Chemical Development, AstraZeneca, Silk Rd Business Park, Macclesfield SK10 2NA (UK)., Grogan G; Department of Chemistry, University of York, Heslington, York, YO10 5DD (UK)., Turner NJ; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester, M1 7DN (UK).
Jazyk: angličtina
Zdroj: ChemCatChem [ChemCatChem] 2015 Feb; Vol. 7 (4), pp. 579-583. Date of Electronic Publication: 2015 Jan 21.
DOI: 10.1002/cctc.201402797
Abstrakt: Although the range of biocatalysts available for the synthesis of enantiomerically pure chiral amines continues to expand, few existing methods provide access to secondary amines. To address this shortcoming, we have over-expressed the gene for an ( R )-imine reductase [( R )-IRED] from Streptomyces sp. GF3587 in Escherichia coli to create a recombinant whole-cell biocatalyst for the asymmetric reduction of prochiral imines. The ( R )-IRED was screened against a panel of cyclic imines and two iminium ions and was shown to possess high catalytic activity and enantioselectivity. Preparative-scale synthesis of the alkaloid ( R )-coniine (90 % yield; 99 % ee ) from the imine precursor was performed on a gram-scale. A homology model of the enzyme active site, based on the structure of a closely related ( R )-IRED from Streptomyces kanamyceticus , was constructed and used to identify potential amino acids as targets for mutagenesis.
Databáze: MEDLINE
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