Chemopreventive effect and angiogenic activity of punicalagin isolated from leaves of Lafoensia pacari A. St.-Hil.

Autor: Carneiro CC; Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil. Electronic address: profacristiene@gmail.com., da Costa Santos S; Instituto de Química, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil., de Souza Lino R Jr; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605050 Goiânia, GO, Brazil., Bara MT; Faculdade de Farmácia, Universidade Federal de Goiás, 74605170 Goiânia, GO, Brazil., Chaibub BA; Faculdade de Farmácia, Universidade Federal de Goiás, 74605170 Goiânia, GO, Brazil., de Melo Reis PR; Laboratório de Estudos Experimentais e Biotecnológicos, Pontifícia Universidade Católica de Goiás, 74605010 Goiânia, GO, Brazil., Chaves DA; Laboratório de Estudos Experimentais e Biotecnológicos, Pontifícia Universidade Católica de Goiás, 74605010 Goiânia, GO, Brazil., da Silva AJ; Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941590 Rio de Janeiro, RJ, Brazil., Silva LS; Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil., de Melo E Silva D; Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil., Chen-Chen L; Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil.
Jazyk: angličtina
Zdroj: Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2016 Nov 01; Vol. 310, pp. 1-8. Date of Electronic Publication: 2016 Aug 18.
DOI: 10.1016/j.taap.2016.08.015
Abstrakt: Punicalagin is the major ellagitannin constituent from leaves of Lafoensia pacari, a Brazilian medicinal plant widely used for the treatment of peptic ulcer and wound healing. Genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of punicalagin were assessed using micronucleus (MN) test and comet assay in mice. Due to the extensive use of L. pacari in the wound healing process, we also assessed the angiogenic activity of punicalagin using the chick chorioallantoic membrane (CAM) angiogenic assay. The highest dose of punicalagin (50mg/kg) showed significant cytotoxic effect by MN test and in the co-treatment with cyclophosphamide (CPA), this cytotoxicity was enhanced. Co-treatment, pre-treatment and post-treatment of punicalagin with CPA led to a significant reduction in the number of DNA breaks and in the frequency of CPA-induced MN, indicating antigenotoxic effect. Using the CAM model, punicalagin exhibited angiogenic activity in all doses mainly at the lowest concentration (12.5μg/μL). Therefore, these findings indicate an effective chemopreventive role of punicalagin and a high capacity to induce DNA repair. Also, the angiogenic activity presented by punicalagin in this study could contribute for the processes of tissue repairing and wound healing.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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