Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria.

Autor: Markakpo US; School of Public Health, University of Ghana, Legon, Ghana. umarkakpo@ug.edu.gh.; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana. umarkakpo@ug.edu.gh., Bosompem KM; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana., Dzodzomenyo M; School of Public Health, University of Ghana, Legon, Ghana., Danso-Appiah A; School of Public Health, University of Ghana, Legon, Ghana., Essuman EE; School of Public Health, University of Ghana, Legon, Ghana., Anyan WK; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana., Suzuki M; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.; Section of Environmental Parasitology, Tokyo Medical and Dental University, Tokyo, Japan., Stephens JK; School of Public Health, University of Ghana, Legon, Ghana., Anim-Baidoo I; School of Allied Health Sciences, University of Ghana, Korlebu, Ghana., Asmah RH; School of Allied Health Sciences, University of Ghana, Korlebu, Ghana., Ofori MF; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana., Madjitey P; School of Public Health, University of Ghana, Legon, Ghana., Danquah JB; School of Public Health, University of Ghana, Legon, Ghana., Frempong NA; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana., Kwofie KD; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana., Amoa-Bosompem M; Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana., Sullivan D Jr; Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Fobil JN; School of Public Health, University of Ghana, Legon, Ghana., Quakyi IA; School of Public Health, University of Ghana, Legon, Ghana.
Jazyk: angličtina
Zdroj: Tropical medicine & international health : TM & IH [Trop Med Int Health] 2016 Oct; Vol. 21 (10), pp. 1263-1271. Date of Electronic Publication: 2016 Aug 22.
DOI: 10.1111/tmi.12744
Abstrakt: Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens.
Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests.
Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines.
Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.
(© 2016 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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