The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing.

Autor:	Pourcelot M; INSERM, UMR_S 1197, Hôpital Paul Brousse, Villejuif, France.; Université Paris-Saclay, Paris, France.; Equipe Labellisée Ligue contre le Cancer, Villejuif, France., Zemirli N; INSERM, UMR_S 1197, Hôpital Paul Brousse, Villejuif, France.; Université Paris-Saclay, Paris, France.; Equipe Labellisée Ligue contre le Cancer, Villejuif, France., Silva Da Costa L; INSERM, UMR_S 1197, Hôpital Paul Brousse, Villejuif, France.; Université Paris-Saclay, Paris, France.; Equipe Labellisée Ligue contre le Cancer, Villejuif, France., Loyant R; INSERM, UMR_S 1197, Hôpital Paul Brousse, Villejuif, France.; Université Paris-Saclay, Paris, France.; Equipe Labellisée Ligue contre le Cancer, Villejuif, France., Garcin D; Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland., Vitour D; ANSES, INRA, ENVA, UPEC, UMR_1161 Virology, LabEx IBEID, Maisons-Alfort, France., Munitic I; Laboratory of Molecular Immunology, Department of Biotechnology, University of Rijeka, Rijeka, Croatia., Vazquez A; INSERM, UMR_S 1197, Hôpital Paul Brousse, Villejuif, France.; Université Paris-Saclay, Paris, France.; Equipe Labellisée Ligue contre le Cancer, Villejuif, France., Arnoult D; INSERM, UMR_S 1197, Hôpital Paul Brousse, Villejuif, France. damien.arnoult@inserm.fr.; Université Paris-Saclay, Paris, France. damien.arnoult@inserm.fr.; Equipe Labellisée Ligue contre le Cancer, Villejuif, France. damien.arnoult@inserm.fr.
Jazyk:	angličtina
Zdroj:	BMC biology [BMC Biol] 2016 Aug 18; Vol. 14, pp. 69. Date of Electronic Publication: 2016 Aug 18.
DOI:	10.1186/s12915-016-0292-z
Abstrakt:	Background: After viral infection and the stimulation of some pattern-recognition receptors, TANK-binding kinase I (TBK1) is activated by K63-linked polyubiquitination followed by trans-autophosphorylation. While the activated TBK1 induces type I interferon production by phosphorylating the transcription factor IRF3, the precise molecular mechanisms underlying TBK1 activation remain unclear. Results: We report here the localization of the ubiquitinated and phosphorylated active form of TBK1 to the Golgi apparatus after the stimulation of RIG-I-like receptors (RLRs) or Toll-like receptor-3 (TLR3), due to TBK1 K63-linked ubiquitination on lysine residues 30 and 401. The ubiquitin-binding protein optineurin (OPTN) recruits ubiquitinated TBK1 to the Golgi apparatus, leading to the formation of complexes in which TBK1 is activated by trans-autophosphorylation. Indeed, OPTN deficiency in various cell lines and primary cells impairs TBK1 targeting to the Golgi apparatus and its activation following RLR or TLR3 stimulation. Interestingly, the Bluetongue virus NS3 protein binds OPTN at the Golgi apparatus, neutralizing its activity and thereby decreasing TBK1 activation and downstream signaling. Conclusions: Our results highlight an unexpected role of the Golgi apparatus in innate immunity as a key subcellular gateway for TBK1 activation after RNA virus infection.
Databáze:	MEDLINE
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