| Autor: |
Georgiou GN; Department of Chemistry and Biological Chemistry, University of Essex, Colchester, England., Morrison IE, Cherry RJ |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1989 Jul 03; Vol. 250 (2), pp. 487-92. |
| DOI: |
10.1016/0014-5793(89)80782-3 |
| Abstrakt: |
Fusion of influenza virus with human erythrocytes at pH 5.2 was followed by fluorescence microscopy using a cooled slow-scan CCD camera. The high sensitivity of the CCD permits repetitive digital imaging of the same cells with minimal photobleaching. The experimental conditions were such that only a small number of virus particles were adsorbed per cell. Quantitative analysis of the data indicated that for most cells only a single fusion event took place. This was, however, sufficient to cause haemolysis within 30 min at 20-22 degrees C for about 60% of cells. There was a highly variable time lag between fusion and haemolysis. The lateral diffusion coefficient of virus particles on the cell surface when bound at pH 7.4 was less than 2 x 10(-13) cm2.s-1. The technique should be of value for more detailed studies of the dynamics of viral and other membrane fusion events. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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