Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.

Autor: Ellebedy AH; Emory Vaccine Center, School of Medicine, Emory University, Atlanta, Georgia, USA.; Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, Georgia, USA., Jackson KJ; Department of Pathology, Stanford University, Stanford, California, USA., Kissick HT; Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, Georgia, USA.; Department of Urology, School of Medicine, Emory University, Atlanta, Georgia, USA., Nakaya HI; Department of Pathology, School of Medicine, Emory University, Atlanta, Georgia, USA.; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil., Davis CW; Emory Vaccine Center, School of Medicine, Emory University, Atlanta, Georgia, USA.; Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, Georgia, USA., Roskin KM; Department of Pathology, Stanford University, Stanford, California, USA., McElroy AK; Department of Pediatrics, School of Medicine, Emory University, Atlanta, Georgia, USA.; Viral Special Pathogens Branch, US Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Oshansky CM; Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA., Elbein R; Division of Infectious Diseases, School of Medicine, Emory University, Atlanta, Georgia, USA., Thomas S; Division of Infectious Diseases, School of Medicine, Emory University, Atlanta, Georgia, USA., Lyon GM; Division of Infectious Diseases, School of Medicine, Emory University, Atlanta, Georgia, USA., Spiropoulou CF; Viral Special Pathogens Branch, US Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Mehta AK; Division of Infectious Diseases, School of Medicine, Emory University, Atlanta, Georgia, USA., Thomas PG; Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA., Boyd SD; Department of Pathology, Stanford University, Stanford, California, USA., Ahmed R; Emory Vaccine Center, School of Medicine, Emory University, Atlanta, Georgia, USA.; Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, Georgia, USA.
Jazyk: angličtina
Zdroj: Nature immunology [Nat Immunol] 2016 Oct; Vol. 17 (10), pp. 1226-34. Date of Electronic Publication: 2016 Aug 15.
DOI: 10.1038/ni.3533
Abstrakt: Antigen-specific B cells bifurcate into antibody-secreting cells (ASCs) and memory B cells (MBCs) after infection or vaccination. ASCs (plasmablasts) have been extensively studied in humans, but less is known about B cells that become activated but do not differentiate into plasmablasts. Here we have defined the phenotype and transcriptional program of a subset of antigen-specific B cells, which we have called 'activated B cells' (ABCs), that were distinct from ASCs and were committed to the MBC lineage. We detected ABCs in humans after infection with Ebola virus or influenza virus and also after vaccination. By simultaneously analyzing antigen-specific ASCs and ABCs in human blood after vaccination against influenza virus, we investigated the clonal overlap and extent of somatic hypermutation (SHM) in the ASC (effector) and ABC (memory) lineages. Longitudinal tracking of vaccination-induced hemagglutinin (HA)-specific clones revealed no overall increase in SHM over time, which suggested that repeated annual immunization might have limitations in enhancing the quality of influenza-virus-specific antibody.
Competing Interests: The authors declare no competing financial interests.
Databáze: MEDLINE
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