Impedimetric immunosensor for detection of cardiovascular disorder risk biomarker.

Autor: Khan R; Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Academy of Scientific and Innovative Research, Jorhat 785006, Assam, India. Electronic address: khan.raju@gmail.com., Pal M; Biotechnology Division, CSIR-North East Institute of Science & Technology, Academy of Scientific and Innovative Research, Jorhat 785006, Assam, India., Kuzikov AV; Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia., Bulko T; Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia., Suprun EV; Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia., Shumyantseva VV; Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2016 Nov 01; Vol. 68, pp. 52-58. Date of Electronic Publication: 2016 May 24.
DOI: 10.1016/j.msec.2016.05.107
Abstrakt: We report the construction and characterization of a novel, level free impedimetric immunosensor for rapid, sensitive and selective detection of myoglobin (Mb). Monoclonal anti-myoglobin (anti-Mb-IgG) antibody was immobilized on screen-printed multiwalled carbon nanotubes electrode for signal amplification without the need of natural enzymes. The fabrication of resulting immunosensor was extensively characterized by using scanning electron microscopy (SEM), fourier transform infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Electrochemical impedance spectroscopy (EIS) technique offered a linear detection range (0.1-90ngmL(-1)) of myoglobin with sensitivity of 0.74kΩngmL(-1) (correlation coefficient, R(2)=0.97) and detection limit of 0.08ngmL(-1) (S/N=3). The mean percentage recovery of Mb in serum samples using this working biosensor is 97.33%. Furthermore, the proposed strategy can be a promising alternative for detection of Mb related cardiovascular disorders.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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